Patterning of peptide nucleic acids using reactive microcontact printing

Alessandro Calabretta, Dorothee Wasserberg, Geertruida A Posthuma-Trumpie, Vinod Subramaniam, Aart van Amerongen, Roberto Corradini, Tullia Tedeschi, Stefano Sforza, David N Reinhoudt, Rosangela Marchelli, Jurriaan Huskens, Pascal Jonkheijm

    Research output: Contribution to JournalArticleAcademicpeer-review


    PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides.

    Original languageEnglish
    Pages (from-to)1536-42
    Number of pages7
    Issue number4
    Publication statusPublished - 15 Feb 2011


    • Microscopy, Fluorescence
    • Peptide Nucleic Acids
    • Journal Article
    • Research Support, Non-U.S. Gov't


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