Pbp, a cell-surface exposed plasminogen binding protein of Bacteroides fragilis.

R. Sijbrandi, M. Stork, S. Luirink, B.R. Otto

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The Gram-negative anaerobic bacterium B. fragilis is a member of the commensal flora of the human intestine, but is also frequently found in severe intra-abdominal infections. Several B. fragilis virulence factors have been implicated in the development of these infections. A B. fragilis protein of circa 60-kDa was identified as a putative plasminogen binding protein (Pbp). The corresponding gene was located, cloned, sequenced and the subcellular localization of the protein was investigated. Pbp was both determined in the outer membrane of B. fragilis and of E. coli that expressed the cloned protein. Protease accessibility studies showed that the protein is expressed at the cell surface. Importantly, we demonstrated that Pbp is sufficient and required for plasminogen binding to whole cells in both E. coli and B. fragilis. Pbp-like proteins were also detected in some other Bacteroides subspecies. The role of this potential B. fragilis virulence factor in pathogenicity is discussed. © 2008 Elsevier Masson SAS. All rights reserved.
Original languageEnglish
Pages (from-to)514-521
Number of pages8
JournalMicrobes and Infection
Volume10
DOIs
Publication statusPublished - 2009

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Bacteroides fragilis
Plasminogen
Carrier Proteins
Virulence Factors
Proteins
Gram-Negative Anaerobic Bacteria
Escherichia coli
Intraabdominal Infections
Bacteroides
Intestines
Virulence
Peptide Hydrolases
Membranes
Infection
Genes

Cite this

@article{7442cd0e20074fbe83fc626c63cbb28a,
title = "Pbp, a cell-surface exposed plasminogen binding protein of Bacteroides fragilis.",
abstract = "The Gram-negative anaerobic bacterium B. fragilis is a member of the commensal flora of the human intestine, but is also frequently found in severe intra-abdominal infections. Several B. fragilis virulence factors have been implicated in the development of these infections. A B. fragilis protein of circa 60-kDa was identified as a putative plasminogen binding protein (Pbp). The corresponding gene was located, cloned, sequenced and the subcellular localization of the protein was investigated. Pbp was both determined in the outer membrane of B. fragilis and of E. coli that expressed the cloned protein. Protease accessibility studies showed that the protein is expressed at the cell surface. Importantly, we demonstrated that Pbp is sufficient and required for plasminogen binding to whole cells in both E. coli and B. fragilis. Pbp-like proteins were also detected in some other Bacteroides subspecies. The role of this potential B. fragilis virulence factor in pathogenicity is discussed. {\circledC} 2008 Elsevier Masson SAS. All rights reserved.",
author = "R. Sijbrandi and M. Stork and S. Luirink and B.R. Otto",
year = "2009",
doi = "10.1016/j.micinf.2008.01.015",
language = "English",
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pages = "514--521",
journal = "Microbes and Infection",
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Pbp, a cell-surface exposed plasminogen binding protein of Bacteroides fragilis. / Sijbrandi, R.; Stork, M.; Luirink, S.; Otto, B.R.

In: Microbes and Infection, Vol. 10, 2009, p. 514-521.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Pbp, a cell-surface exposed plasminogen binding protein of Bacteroides fragilis.

AU - Sijbrandi, R.

AU - Stork, M.

AU - Luirink, S.

AU - Otto, B.R.

PY - 2009

Y1 - 2009

N2 - The Gram-negative anaerobic bacterium B. fragilis is a member of the commensal flora of the human intestine, but is also frequently found in severe intra-abdominal infections. Several B. fragilis virulence factors have been implicated in the development of these infections. A B. fragilis protein of circa 60-kDa was identified as a putative plasminogen binding protein (Pbp). The corresponding gene was located, cloned, sequenced and the subcellular localization of the protein was investigated. Pbp was both determined in the outer membrane of B. fragilis and of E. coli that expressed the cloned protein. Protease accessibility studies showed that the protein is expressed at the cell surface. Importantly, we demonstrated that Pbp is sufficient and required for plasminogen binding to whole cells in both E. coli and B. fragilis. Pbp-like proteins were also detected in some other Bacteroides subspecies. The role of this potential B. fragilis virulence factor in pathogenicity is discussed. © 2008 Elsevier Masson SAS. All rights reserved.

AB - The Gram-negative anaerobic bacterium B. fragilis is a member of the commensal flora of the human intestine, but is also frequently found in severe intra-abdominal infections. Several B. fragilis virulence factors have been implicated in the development of these infections. A B. fragilis protein of circa 60-kDa was identified as a putative plasminogen binding protein (Pbp). The corresponding gene was located, cloned, sequenced and the subcellular localization of the protein was investigated. Pbp was both determined in the outer membrane of B. fragilis and of E. coli that expressed the cloned protein. Protease accessibility studies showed that the protein is expressed at the cell surface. Importantly, we demonstrated that Pbp is sufficient and required for plasminogen binding to whole cells in both E. coli and B. fragilis. Pbp-like proteins were also detected in some other Bacteroides subspecies. The role of this potential B. fragilis virulence factor in pathogenicity is discussed. © 2008 Elsevier Masson SAS. All rights reserved.

U2 - 10.1016/j.micinf.2008.01.015

DO - 10.1016/j.micinf.2008.01.015

M3 - Article

VL - 10

SP - 514

EP - 521

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

ER -