Abstract
Aim: To evaluate the effect of bone morphogenetic protein 2 (BMP-2) incorporated biomimetic calcium phosphate (BMP-2/BioCaP) in conjunction with barrier membrane on periodontal regeneration in chronic periodontitis experimental model.
Material and Methods: Chronic periodontitis experimental model with critical-sized supra-alveolar defects was created in 15 dogs’ mandibles. After the initial periodontal therapy, the defects were randomly assigned to the following groups: (a) control; (b) barrier membrane; (c) deproteinized bovine bone mineral + barrier membrane; (d) BioCaP + barrier membrane and (e) BMP-2/BioCaP + barrier membrane (6 quadrants with 18 teeth per group). Eight weeks later, clinical examinations, micro-CT, and histomorphometric analyses were performed.
Results: Clinical examinations, including plaque index, bleeding index, and probing depth, were similar for all groups. In contrast, the clinical attachment loss was significantly lower in defects grafted with BMP-2/BioCaP and barrier membrane. The micro-CT results showed that the height of mineralized tissue in defects grafted with BMP-2/BioCaP and barrier membrane was significantly higher. For histometric analysis, the defects grafted with BMP-2/BioCaP and barrier membrane exhibited significantly more connective tissue height, new cementum height, new bone height and area, as well as less down-growth of junctional epithelium. Conclusion: BMP-2/BioCaP could be a promising bone substitute for periodontal regeneration.
Material and Methods: Chronic periodontitis experimental model with critical-sized supra-alveolar defects was created in 15 dogs’ mandibles. After the initial periodontal therapy, the defects were randomly assigned to the following groups: (a) control; (b) barrier membrane; (c) deproteinized bovine bone mineral + barrier membrane; (d) BioCaP + barrier membrane and (e) BMP-2/BioCaP + barrier membrane (6 quadrants with 18 teeth per group). Eight weeks later, clinical examinations, micro-CT, and histomorphometric analyses were performed.
Results: Clinical examinations, including plaque index, bleeding index, and probing depth, were similar for all groups. In contrast, the clinical attachment loss was significantly lower in defects grafted with BMP-2/BioCaP and barrier membrane. The micro-CT results showed that the height of mineralized tissue in defects grafted with BMP-2/BioCaP and barrier membrane was significantly higher. For histometric analysis, the defects grafted with BMP-2/BioCaP and barrier membrane exhibited significantly more connective tissue height, new cementum height, new bone height and area, as well as less down-growth of junctional epithelium. Conclusion: BMP-2/BioCaP could be a promising bone substitute for periodontal regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 1254-1263 |
| Journal | Journal of Clinical Periodontology |
| Volume | 46 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2019 |
Funding
Funding information This project was supported by the Natural Science Foundation of Shandong Province (No.ZR2014HP019), Medical Science and Technology Development Plan of Shandong Province (No.2015WS0041) and Shandong Taishan Scholar Program to Dr. Yuelian Liu. The authors also gave appreciate to Geistlich Pharma AG for the free-issue materials, including barrier membrane and deproteinized bovine bone mineral.
| Funders | Funder number |
|---|---|
| Geistlich Pharma AG | |
| Department of Science and Technology of Shandong Province | 2015WS0041 |
| Natural Science Foundation of Shandong Province | No.ZR2014HP019 |
| Taishan Scholar Foundation of Shandong Province |