Abstract
Objective: Periostin (PN), a major matricellular periodontal ligament (PDL) protein, modulates the remodeling of the PDL and bone, especially under mechanical stress. This study investigated the requirement of PN-integrin signaling in force-induced expression of transforming growth factor-beta 1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) in human PDL stem cells (hPDLSCs). Methods: Cells were stimulated with intermittent compressive force (ICF) using computerized controlled apparatus. Cell migration was examined using in vitro scratch assay. The mRNA expression was examined using real-time polymerase chain reaction. The protein expression was determined using immunofluorescent staining and western blot analysis. Results: Stimulation with ICF for 24 h increased the expression of PN, TGF-β1, and α-SMA, along with increased SMAD2/3 phosphorylation. Knockdown of POSTN (PN gene) decreased the protein levels of TGF-β1 and pSMAD2/3 upon force stimulation. POSTN knockdown of hPDLSCs resulted in delayed cell migration, as determined by a scratch assay. However, migration improved after seeding these knockdown cells on pre-PN-coated surfaces. Further, the knockdown of αVβ5 significantly attenuated the force-induced TGF-β1 expression. Conclusion: Our findings indicate the importance of PN-αVβ5 interactions in ICF-induced TGF-β1 signaling and the expression of α-SMA. Findings support the critical role of PN in maintaining the PDL's tissue integrity and homeostasis.
Original language | English |
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Pages (from-to) | 2570-2579 |
Number of pages | 10 |
Journal | Oral Diseases |
Volume | 30 |
Issue number | 4 |
Early online date | 19 Jul 2023 |
DOIs | |
Publication status | Published - May 2024 |
Bibliographical note
Publisher Copyright:© 2023 Wiley Periodicals LLC.
Funding
This work was supported by the NSRF via The Program Management Unit for Human Resources & Institutional Development, Research and Innovation (B16F640118 to T.O.), and the Second Century Fund, Chulalongkorn University (C2F to D.N.).
Funders | Funder number |
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NSRF | B16F640118 |
Second Century Fund | |
Chulalongkorn University |
Keywords
- integrin
- mechanical force
- periodontal ligament cells
- periostin