Peroxisomes from the Heavy Mitochondrial Fraction: Isolation by Zonal Free Flow Electrophoresis and Quantitative Mass Spectrometrical Characterization

M. Islinger, K.W. Li, M. Loos, S. Liebler, S. Angermuller, C Eckerskorn, G. Weber, A. Abdolzade, A. Volkl

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Peroxisomes are a heterogeneous group of organelles fulfilling reactions in a variety of metabolic pathways. To investigate if functionally different subpopulations can be found within a single tissue, peroxisomes from the heavy mitochondrial fraction (HM-Po) of the rat liver were isolated and compared to "classic" peroxisomes from the light mitochondrial fraction (LM-Po) using iTRAQ tandem mass spectrometry. Peroxisomes represent only a minor although significant proportion of the heavy mitochondrial fraction (2700g
Original languageEnglish
Pages (from-to)113-124
JournalJournal of Proteome Research
Volume9
Issue number1
DOIs
Publication statusPublished - 2010

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