Phase-stabilized optical frequency domain imaging at 1-mu m for the measurement of blood flow in the human choroid

B. Braaf, K.A. Vermeer, V.A.D.P. Sicam, E. Zeeburg, J.C. van Meurs, J.F. de Boer

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In optical frequency domain imaging (OFDI) the measurement of interference fringes is not exactly reproducible due to small instabilities in the swept-source laser, the interferometer and the data-acquisition hardware. The resulting variation in wavenumber sampling makes phase-resolved detection and the removal of fixed-pattern noise challenging in OFDI. In this paper this problem is solved by a new post-processing method in which interference fringes are resampled to the exact same wavenumber space using a simultaneously recorded calibration signal. This method is implemented in a high-speed (100 kHz) high-resolution (6.5 μm) OFDI system at 1-μm and is used for the removal of fixed-pattern noise artifacts and for phase-resolved blood flow measurements in the human choroid. The system performed close to the shot-noise limit (<1dB) with a sensitivity of 99.1 dB for a 1.7 mW sample arm power. Suppression of fixed-pattern noise artifacts is shown up to 39.0 dB which effectively removes all artifacts from the OFDI-images. The clinical potential of the system is shown by the detection of choroidal blood flow in a healthy volunteer and the detection of tissue reperfusion in a patient after a retinal pigment epithelium and choroid transplantation. © 2011 Optical Society of America.
Original languageEnglish
Pages (from-to)20886-20903
Number of pages18
JournalOptics Express
Issue number21
Publication statusPublished - 2011


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