Phosphoglycerate kinase acts as a futile cycle at high temperature

Theresa Kouril, Johann J Eicher, Bettina Siebers, J.L. Snoep

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Abstract

In (hyper)thermophilic organisms metabolic processes have to be adapted to function optimally at high temperature. We
compared the gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate
at 30 C and at 70 C. At 30 C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with
phosphoglycerate kinase, but at 70 C, 1,3-bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate,
effectively turning the phosphoglycerate kinase into a futile cycle. When phosphoglycerate kinase was incubated together
with glyceraldehyde 3-phosphate dehydrogenase it was possible to convert 3-phosphoglycerate to glyceraldehyde 3-
phosphate, both at 30 C and at 70 C, however, at 70 C only low concentrations of product were observed due to thermal
instability of glyceraldehyde 3-phosphate. Thus, thermolabile intermediates challenge central metabolic reactions and
require special adaptation strategies for life at high temperature.
Original languageEnglish
Article number000542
Pages (from-to)1604-1612
Number of pages9
JournalMicrobiology
Volume163
Issue number11
Early online date6 Oct 2017
DOIs
Publication statusPublished - 1 Nov 2017

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