Photoinduced transformation of UVR8 monitored by vibrational and fluorescence spectroscopy

M. Heilmann, J.M. Christie, J.T.M. Kennis, G.I. Jenkins, T. Mathes

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Tryptophan residues at the dimer interface of the plant photoreceptor UVR8 promote monomerisation after UV-B absorption via a so far unknown mechanism. Using FTIR spectroscopy we assign light-induced structural transitions of UVR8 mainly to amino acid side chains without major transformations of the secondary structure of the physiologically relevant C-terminal extension. Additionally, we assign the monomerisation associated increase and red shift of the UVR8 tryptophan emission to a photoinduced rearrangement of tryptophan side chains and a relocation of the aspartic acid residues D96 and D107, respectively. By illumination dependent emission spectroscopy we furthermore determined the quantum yield of photoinduced monomerisation to 20 ± 8%. This journal is
Original languageEnglish
Pages (from-to)252-257
JournalPhotochemical and Photobiological Sciences
Volume14
Issue number2
DOIs
Publication statusPublished - 2015

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