Phycocyanin: One Complex, Two States, Two Functions

Michal Gwizdala, Tjaart P.J. Krüger, Md Wahadoszamen, J. Michael Gruber, Rienk Van Grondelle

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Solar energy captured by pigments embedded in light-harvesting complexes can be transferred to neighboring pigments, dissipated, or emitted as fluorescence. Only when it reaches a reaction center is the excitation energy stabilized in the form of a charge separation and converted into chemical energy. Well-directed and regulated energy transfer within the network of pigments is therefore of crucial importance for the success of the photosynthetic processes. Using single-molecule spectroscopy, we show that phycocyanin can dynamically switch between two spectrally distinct states originating from two different conformations. Unexpectedly, one of the two states has a red-shifted emission spectrum. This state is not involved in energy dissipation; instead, we propose that it is involved in direct energy transfer to photosystem I. Finally, our findings suggest that the function of linker proteins in phycobilisomes is to stabilize one state or the other, thus controlling the light-harvesting functions of phycocyanin.

Original languageEnglish
Pages (from-to)1365-1371
Number of pages7
JournalJournal of Physical Chemistry Letters
Volume9
Issue number6
DOIs
Publication statusPublished - 15 Mar 2018

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Phycocyanin
pigments
Pigments
Energy transfer
energy transfer
Photosystem I Protein Complex
chemical energy
Excitation energy
solar energy
polarization (charge separation)
Solar energy
Conformations
Energy dissipation
emission spectra
energy dissipation
Fluorescence
Switches
Spectroscopy
proteins
Proteins

Cite this

Gwizdala, Michal ; Krüger, Tjaart P.J. ; Wahadoszamen, Md ; Gruber, J. Michael ; Van Grondelle, Rienk. / Phycocyanin: One Complex, Two States, Two Functions. In: Journal of Physical Chemistry Letters. 2018 ; Vol. 9, No. 6. pp. 1365-1371.
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abstract = "Solar energy captured by pigments embedded in light-harvesting complexes can be transferred to neighboring pigments, dissipated, or emitted as fluorescence. Only when it reaches a reaction center is the excitation energy stabilized in the form of a charge separation and converted into chemical energy. Well-directed and regulated energy transfer within the network of pigments is therefore of crucial importance for the success of the photosynthetic processes. Using single-molecule spectroscopy, we show that phycocyanin can dynamically switch between two spectrally distinct states originating from two different conformations. Unexpectedly, one of the two states has a red-shifted emission spectrum. This state is not involved in energy dissipation; instead, we propose that it is involved in direct energy transfer to photosystem I. Finally, our findings suggest that the function of linker proteins in phycobilisomes is to stabilize one state or the other, thus controlling the light-harvesting functions of phycocyanin.",
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Phycocyanin: One Complex, Two States, Two Functions. / Gwizdala, Michal; Krüger, Tjaart P.J.; Wahadoszamen, Md; Gruber, J. Michael; Van Grondelle, Rienk.

In: Journal of Physical Chemistry Letters, Vol. 9, No. 6, 15.03.2018, p. 1365-1371.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Gwizdala, Michal

AU - Krüger, Tjaart P.J.

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AU - Gruber, J. Michael

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AB - Solar energy captured by pigments embedded in light-harvesting complexes can be transferred to neighboring pigments, dissipated, or emitted as fluorescence. Only when it reaches a reaction center is the excitation energy stabilized in the form of a charge separation and converted into chemical energy. Well-directed and regulated energy transfer within the network of pigments is therefore of crucial importance for the success of the photosynthetic processes. Using single-molecule spectroscopy, we show that phycocyanin can dynamically switch between two spectrally distinct states originating from two different conformations. Unexpectedly, one of the two states has a red-shifted emission spectrum. This state is not involved in energy dissipation; instead, we propose that it is involved in direct energy transfer to photosystem I. Finally, our findings suggest that the function of linker proteins in phycobilisomes is to stabilize one state or the other, thus controlling the light-harvesting functions of phycocyanin.

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