Abstract
The physical biology of G protein-coupled receptor (GPCR) signalling can be inferred from imaging of single molecules and single living cells. In this opinion paper, we highlight recent developments in technologies to study GPCR signalling in vitro and in cyto. We start from mobility and localisation characteristics of single receptors in membranes. Subsequently, we discuss the kinetics of shifts in receptor-conformation equilibrium due to allosteric binding events and G protein activation. We continue with recent insights into downstream signalling and the role of delayed negative feedback to suppress GPCR signalling. Finally, we discuss new strategies to reveal how the multiplex signalling responses of cells to ligand mixtures, mediated by their entire receptor arsenal, can be disentangled, using single-cell data.
| Original language | English |
|---|---|
| Pages (from-to) | 204-211 |
| Number of pages | 8 |
| Journal | Current Opinion in Structural Biology |
| Volume | 55 |
| DOIs | |
| Publication status | Published - 1 Apr 2019 |
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Physical biology of GPCR signalling dynamics inferred from fluorescence spectroscopy and imaging. / Chavez-Abiega, Sergei; Goedhart, Joachim; Bruggeman, Frank Johannes.
In: Current Opinion in Structural Biology, Vol. 55, 01.04.2019, p. 204-211.Research output: Contribution to Journal › Review article › Academic › peer-review
TY - JOUR
T1 - Physical biology of GPCR signalling dynamics inferred from fluorescence spectroscopy and imaging
AU - Chavez-Abiega, Sergei
AU - Goedhart, Joachim
AU - Bruggeman, Frank Johannes
PY - 2019/4/1
Y1 - 2019/4/1
N2 - The physical biology of G protein-coupled receptor (GPCR) signalling can be inferred from imaging of single molecules and single living cells. In this opinion paper, we highlight recent developments in technologies to study GPCR signalling in vitro and in cyto. We start from mobility and localisation characteristics of single receptors in membranes. Subsequently, we discuss the kinetics of shifts in receptor-conformation equilibrium due to allosteric binding events and G protein activation. We continue with recent insights into downstream signalling and the role of delayed negative feedback to suppress GPCR signalling. Finally, we discuss new strategies to reveal how the multiplex signalling responses of cells to ligand mixtures, mediated by their entire receptor arsenal, can be disentangled, using single-cell data.
AB - The physical biology of G protein-coupled receptor (GPCR) signalling can be inferred from imaging of single molecules and single living cells. In this opinion paper, we highlight recent developments in technologies to study GPCR signalling in vitro and in cyto. We start from mobility and localisation characteristics of single receptors in membranes. Subsequently, we discuss the kinetics of shifts in receptor-conformation equilibrium due to allosteric binding events and G protein activation. We continue with recent insights into downstream signalling and the role of delayed negative feedback to suppress GPCR signalling. Finally, we discuss new strategies to reveal how the multiplex signalling responses of cells to ligand mixtures, mediated by their entire receptor arsenal, can be disentangled, using single-cell data.
UR - http://www.scopus.com/inward/record.url?scp=85068772898&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85068772898&partnerID=8YFLogxK
U2 - 10.1016/j.sbi.2019.05.007
DO - 10.1016/j.sbi.2019.05.007
M3 - Review article
VL - 55
SP - 204
EP - 211
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
SN - 0959-440X
ER -