Physical biology of GPCR signalling dynamics inferred from fluorescence spectroscopy and imaging

Sergei Chavez-Abiega; Joachim Goedhart; Frank Johannes Bruggeman

doi:10.1016/j.sbi.2019.05.007

Physical biology of GPCR signalling dynamics inferred from fluorescence spectroscopy and imaging

Sergei Chavez-Abiega, Joachim Goedhart, Frank Johannes Bruggeman

Research output: Contribution to Journal › Review article › Academic › peer-review

Abstract

The physical biology of G protein-coupled receptor (GPCR) signalling can be inferred from imaging of single molecules and single living cells. In this opinion paper, we highlight recent developments in technologies to study GPCR signalling in vitro and in cyto. We start from mobility and localisation characteristics of single receptors in membranes. Subsequently, we discuss the kinetics of shifts in receptor-conformation equilibrium due to allosteric binding events and G protein activation. We continue with recent insights into downstream signalling and the role of delayed negative feedback to suppress GPCR signalling. Finally, we discuss new strategies to reveal how the multiplex signalling responses of cells to ligand mixtures, mediated by their entire receptor arsenal, can be disentangled, using single-cell data.

Original language	English
Pages (from-to)	204-211
Number of pages	8
Journal	Current Opinion in Structural Biology
Volume	55
DOIs	https://doi.org/10.1016/j.sbi.2019.05.007
Publication status	Published - 1 Apr 2019

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title = "Physical biology of GPCR signalling dynamics inferred from fluorescence spectroscopy and imaging",

abstract = "The physical biology of G protein-coupled receptor (GPCR) signalling can be inferred from imaging of single molecules and single living cells. In this opinion paper, we highlight recent developments in technologies to study GPCR signalling in vitro and in cyto. We start from mobility and localisation characteristics of single receptors in membranes. Subsequently, we discuss the kinetics of shifts in receptor-conformation equilibrium due to allosteric binding events and G protein activation. We continue with recent insights into downstream signalling and the role of delayed negative feedback to suppress GPCR signalling. Finally, we discuss new strategies to reveal how the multiplex signalling responses of cells to ligand mixtures, mediated by their entire receptor arsenal, can be disentangled, using single-cell data.",

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AB - The physical biology of G protein-coupled receptor (GPCR) signalling can be inferred from imaging of single molecules and single living cells. In this opinion paper, we highlight recent developments in technologies to study GPCR signalling in vitro and in cyto. We start from mobility and localisation characteristics of single receptors in membranes. Subsequently, we discuss the kinetics of shifts in receptor-conformation equilibrium due to allosteric binding events and G protein activation. We continue with recent insights into downstream signalling and the role of delayed negative feedback to suppress GPCR signalling. Finally, we discuss new strategies to reveal how the multiplex signalling responses of cells to ligand mixtures, mediated by their entire receptor arsenal, can be disentangled, using single-cell data.

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