Pigment-Protein Interactions in Phytochromes Probed by Fluorescence Line Narrowing Spectroscopy

J.B. Nieder, E.A Stojkovic, K. Moffat, K.T. Forest, T. Lamparter, R. Bittl, J.T.M. Kennis

Research output: Contribution to JournalArticleAcademicpeer-review


Fluorescence line narrowing (FLN) spectroscopy was used to study bacteriophytochromes and variants from various species in their red-absorbing Pr ground state, including phytochromes Agp1 from Agrobacterium tumefaciens, DrBphP from Deinococcus radiodurans, and RpBphP2 and RpBphP3 from Rhodopseudomonas palustris. A species-dependent narrowing of the fluorescence emission bands is observed. The results suggest varied pigment-protein interactions, possibly connected to chromophore mobility or extended water pyrrole networks inside of the differing binding pockets. Solvent water isotope exchange from H
Original languageEnglish
Pages (from-to)14940-14950
JournalJournal of Physical Chemistry B
Issue number48
Publication statusPublished - 2013


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