Plasma cells are not restricted to the CD27+ phenotype: characterization of CD27-CD43+ antibody-secreting cells

Kris Covens; Bert Verbinnen; Britt G. de Jong; Leen Moens; Greet Wuyts; Geert Verheyen; Kris Nys; Jonathan Cremer; Stijn Smulders; Rik Schrijvers; Andreas Weinhäusel; Séverine Vermeire; Patrick Verschueren; Ellen De Langhe; Jacques J.M. van Dongen; Menno C. van Zelm; Xavier Bossuyt

doi:10.3389/fimmu.2023.1165936

Plasma cells are not restricted to the CD27+ phenotype: characterization of CD27-CD43+ antibody-secreting cells

Kris Covens, Bert Verbinnen, Britt G. de Jong, Leen Moens, Greet Wuyts, Geert Verheyen, Kris Nys, Jonathan Cremer, Stijn Smulders, Rik Schrijvers, Andreas Weinhäusel, Séverine Vermeire, Patrick Verschueren, Ellen De Langhe, Jacques J.M. van Dongen, Menno C. van Zelm, Xavier Bossuyt^*

^*Corresponding author for this work

Periodontology

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Circulating antibody-secreting cells are present in the peripheral blood of healthy individuals reflecting the continued activity of the humoral immune system. Antibody-secreting cells typically express CD27. Here we describe and characterize a small population of antibody-secreting class switched CD19+CD43+ B cells that lack expression of CD27 in the peripheral blood of healthy subjects. In this study, we characterized CD27-CD43+ cells. We demonstrate that class-switched CD27-CD43+ B cells possess characteristics of conventional plasmablasts as they spontaneously secrete antibodies, are morphologically similar to antibody-secreting cells, show downregulation of B cell differentiation markers, and have a gene expression profile related to conventional plasmablasts. Despite these similarities, we observed differences in IgA and IgG subclass distribution, expression of homing markers, replication history, frequency of somatic hypermutation, immunoglobulin repertoire, gene expression related to Toll-like receptors, cytokines, and cytokine receptors, and antibody response to vaccination. Their frequency is altered in immune-mediated disorders. Conclusion: we characterized CD27-CD43+ cells as antibody-secreting cells with differences in function and homing potential as compared to conventional CD27+ antibody-secreting cells.

Original language	English
Article number	1165936
Pages (from-to)	1-16
Number of pages	16
Journal	Frontiers in Immunology
Volume	14
DOIs	https://doi.org/10.3389/fimmu.2023.1165936
Publication status	Published - 10 Jul 2023

Bibliographical note

Funding Information:
This study was supported by a research grant from the Research Council of the KU Leuven (GOA/13/013). KC and BV were supported through a grant from the Flemish Institute for Innovation through Science and Technology. Acknowledgments

Publisher Copyright:
Copyright © 2023 Covens, Verbinnen, de Jong, Moens, Wuyts, Verheyen, Nys, Cremer, Smulders, Schrijvers, Weinhäusel, Vermeire, Verschueren, Langhe, van Dongen, van Zelm and Bossuyt.

Funding

This study was supported by a research grant from the Research Council of the KU Leuven (GOA/13/013). KC and BV were supported through a grant from the Flemish Institute for Innovation through Science and Technology. Acknowledgments

Funders	Funder number
Flemish Institute for Innovation
KU Leuven	GOA/13/013
KU Leuven

Keywords

antibody secreting cells
B lymphocyte
CD27
CD43
plasmablast
somatic hypermutation

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10.3389/fimmu.2023.1165936

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Covens, K., Verbinnen, B., de Jong, B. G., Moens, L., Wuyts, G., Verheyen, G., Nys, K., Cremer, J., Smulders, S., Schrijvers, R., Weinhäusel, A., Vermeire, S., Verschueren, P., Langhe, E. D., van Dongen, J. J. M., van Zelm, M. C., & Bossuyt, X. (2023). Plasma cells are not restricted to the CD27+ phenotype: characterization of CD27-CD43+ antibody-secreting cells. Frontiers in Immunology, 14, 1-16. Article 1165936. https://doi.org/10.3389/fimmu.2023.1165936

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title = "Plasma cells are not restricted to the CD27+ phenotype: characterization of CD27-CD43+ antibody-secreting cells",

abstract = "Circulating antibody-secreting cells are present in the peripheral blood of healthy individuals reflecting the continued activity of the humoral immune system. Antibody-secreting cells typically express CD27. Here we describe and characterize a small population of antibody-secreting class switched CD19+CD43+ B cells that lack expression of CD27 in the peripheral blood of healthy subjects. In this study, we characterized CD27-CD43+ cells. We demonstrate that class-switched CD27-CD43+ B cells possess characteristics of conventional plasmablasts as they spontaneously secrete antibodies, are morphologically similar to antibody-secreting cells, show downregulation of B cell differentiation markers, and have a gene expression profile related to conventional plasmablasts. Despite these similarities, we observed differences in IgA and IgG subclass distribution, expression of homing markers, replication history, frequency of somatic hypermutation, immunoglobulin repertoire, gene expression related to Toll-like receptors, cytokines, and cytokine receptors, and antibody response to vaccination. Their frequency is altered in immune-mediated disorders. Conclusion: we characterized CD27-CD43+ cells as antibody-secreting cells with differences in function and homing potential as compared to conventional CD27+ antibody-secreting cells.",

keywords = "antibody secreting cells, B lymphocyte, CD27, CD43, plasmablast, somatic hypermutation",

author = "Kris Covens and Bert Verbinnen and {de Jong}, {Britt G.} and Leen Moens and Greet Wuyts and Geert Verheyen and Kris Nys and Jonathan Cremer and Stijn Smulders and Rik Schrijvers and Andreas Weinh{\"a}usel and S{\'e}verine Vermeire and Patrick Verschueren and Langhe, {Ellen De} and {van Dongen}, {Jacques J.M.} and {van Zelm}, {Menno C.} and Xavier Bossuyt",

note = "Funding Information: This study was supported by a research grant from the Research Council of the KU Leuven (GOA/13/013). KC and BV were supported through a grant from the Flemish Institute for Innovation through Science and Technology. Acknowledgments Publisher Copyright: Copyright {\textcopyright} 2023 Covens, Verbinnen, de Jong, Moens, Wuyts, Verheyen, Nys, Cremer, Smulders, Schrijvers, Weinh{\"a}usel, Vermeire, Verschueren, Langhe, van Dongen, van Zelm and Bossuyt.",

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doi = "10.3389/fimmu.2023.1165936",

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Covens, K, Verbinnen, B, de Jong, BG, Moens, L, Wuyts, G, Verheyen, G, Nys, K, Cremer, J, Smulders, S, Schrijvers, R, Weinhäusel, A, Vermeire, S, Verschueren, P, Langhe, ED, van Dongen, JJM, van Zelm, MC & Bossuyt, X 2023, 'Plasma cells are not restricted to the CD27+ phenotype: characterization of CD27-CD43+ antibody-secreting cells', Frontiers in Immunology, vol. 14, 1165936, pp. 1-16. https://doi.org/10.3389/fimmu.2023.1165936

TY - JOUR

T1 - Plasma cells are not restricted to the CD27+ phenotype

T2 - characterization of CD27-CD43+ antibody-secreting cells

AU - Covens, Kris

AU - Verbinnen, Bert

AU - de Jong, Britt G.

AU - Moens, Leen

AU - Wuyts, Greet

AU - Verheyen, Geert

AU - Nys, Kris

AU - Cremer, Jonathan

AU - Smulders, Stijn

AU - Schrijvers, Rik

AU - Weinhäusel, Andreas

AU - Vermeire, Séverine

AU - Verschueren, Patrick

AU - Langhe, Ellen De

AU - van Dongen, Jacques J.M.

AU - van Zelm, Menno C.

AU - Bossuyt, Xavier

N1 - Funding Information: This study was supported by a research grant from the Research Council of the KU Leuven (GOA/13/013). KC and BV were supported through a grant from the Flemish Institute for Innovation through Science and Technology. Acknowledgments Publisher Copyright: Copyright © 2023 Covens, Verbinnen, de Jong, Moens, Wuyts, Verheyen, Nys, Cremer, Smulders, Schrijvers, Weinhäusel, Vermeire, Verschueren, Langhe, van Dongen, van Zelm and Bossuyt.

PY - 2023/7/10

Y1 - 2023/7/10

N2 - Circulating antibody-secreting cells are present in the peripheral blood of healthy individuals reflecting the continued activity of the humoral immune system. Antibody-secreting cells typically express CD27. Here we describe and characterize a small population of antibody-secreting class switched CD19+CD43+ B cells that lack expression of CD27 in the peripheral blood of healthy subjects. In this study, we characterized CD27-CD43+ cells. We demonstrate that class-switched CD27-CD43+ B cells possess characteristics of conventional plasmablasts as they spontaneously secrete antibodies, are morphologically similar to antibody-secreting cells, show downregulation of B cell differentiation markers, and have a gene expression profile related to conventional plasmablasts. Despite these similarities, we observed differences in IgA and IgG subclass distribution, expression of homing markers, replication history, frequency of somatic hypermutation, immunoglobulin repertoire, gene expression related to Toll-like receptors, cytokines, and cytokine receptors, and antibody response to vaccination. Their frequency is altered in immune-mediated disorders. Conclusion: we characterized CD27-CD43+ cells as antibody-secreting cells with differences in function and homing potential as compared to conventional CD27+ antibody-secreting cells.

AB - Circulating antibody-secreting cells are present in the peripheral blood of healthy individuals reflecting the continued activity of the humoral immune system. Antibody-secreting cells typically express CD27. Here we describe and characterize a small population of antibody-secreting class switched CD19+CD43+ B cells that lack expression of CD27 in the peripheral blood of healthy subjects. In this study, we characterized CD27-CD43+ cells. We demonstrate that class-switched CD27-CD43+ B cells possess characteristics of conventional plasmablasts as they spontaneously secrete antibodies, are morphologically similar to antibody-secreting cells, show downregulation of B cell differentiation markers, and have a gene expression profile related to conventional plasmablasts. Despite these similarities, we observed differences in IgA and IgG subclass distribution, expression of homing markers, replication history, frequency of somatic hypermutation, immunoglobulin repertoire, gene expression related to Toll-like receptors, cytokines, and cytokine receptors, and antibody response to vaccination. Their frequency is altered in immune-mediated disorders. Conclusion: we characterized CD27-CD43+ cells as antibody-secreting cells with differences in function and homing potential as compared to conventional CD27+ antibody-secreting cells.

KW - antibody secreting cells

KW - B lymphocyte

KW - CD27

KW - CD43

KW - plasmablast

KW - somatic hypermutation

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ER -

Plasma cells are not restricted to the CD27+ phenotype: characterization of CD27-CD43+ antibody-secreting cells

Abstract

Bibliographical note

Funding

Keywords

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