Plastic expression of seminal fluid protein genes in a simultaneously hermaphroditic snail

Yumi Nakadera, Athina Giannakara, Steven A Ramm

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The eukaryotic RNA exosome is an essential, multi-subunit complex that catalyzes RNA turnover, maturation, and quality control processes. Its non-catalytic donut-shaped core includes 9 subunits that associate with the 3' to 5' exoribonucleases Rrp6, and Rrp44/Dis3, a subunit that also catalyzes endoribonuclease activity. Although recent structures and biochemical studies of RNA bound exosomes from S. cerevisiae revealed that the Exo9 central channel guides RNA to either Rrp6 or Rrp44 using partially overlapping and mutually exclusive paths, several issues related to RNA recruitment remain. Here, we identify activities for the highly basic Rrp6 C-terminal tail that we term the 'lasso' because it binds RNA and stimulates ribonuclease activities associated with Rrp44 and Rrp6 within the 11-subunit nuclear exosome. Stimulation is dependent on the Exo9 central channel, and the lasso contributes to degradation and processing activities of exosome substrates in vitro and in vivo. Finally, we present evidence that the Rrp6 lasso may be a conserved feature of the eukaryotic RNA exosome.
Original languageEnglish
Article numberarz027
Pages (from-to)904–913
Number of pages10
JournalBehavioral Ecology
Volume30
Issue number4
Early online date18 Mar 2019
DOIs
Publication statusPublished - Jul 2019

Fingerprint

exosomes
snail
snails
RNA
plastics
plastic
protein
fluid
gene
genes
proteins
exoribonucleases
endoribonucleases
doughnuts
ribonucleases
fluids
quality control
maturation
turnover
tail

Cite this

Nakadera, Yumi ; Giannakara, Athina ; Ramm, Steven A. / Plastic expression of seminal fluid protein genes in a simultaneously hermaphroditic snail. In: Behavioral Ecology. 2019 ; Vol. 30, No. 4. pp. 904–913.
@article{5dfd3f89f0b14acf8fb99580b4e2640d,
title = "Plastic expression of seminal fluid protein genes in a simultaneously hermaphroditic snail",
abstract = "The eukaryotic RNA exosome is an essential, multi-subunit complex that catalyzes RNA turnover, maturation, and quality control processes. Its non-catalytic donut-shaped core includes 9 subunits that associate with the 3' to 5' exoribonucleases Rrp6, and Rrp44/Dis3, a subunit that also catalyzes endoribonuclease activity. Although recent structures and biochemical studies of RNA bound exosomes from S. cerevisiae revealed that the Exo9 central channel guides RNA to either Rrp6 or Rrp44 using partially overlapping and mutually exclusive paths, several issues related to RNA recruitment remain. Here, we identify activities for the highly basic Rrp6 C-terminal tail that we term the 'lasso' because it binds RNA and stimulates ribonuclease activities associated with Rrp44 and Rrp6 within the 11-subunit nuclear exosome. Stimulation is dependent on the Exo9 central channel, and the lasso contributes to degradation and processing activities of exosome substrates in vitro and in vivo. Finally, we present evidence that the Rrp6 lasso may be a conserved feature of the eukaryotic RNA exosome.",
author = "Yumi Nakadera and Athina Giannakara and Ramm, {Steven A}",
year = "2019",
month = "7",
doi = "10.1093/beheco/arz027",
language = "English",
volume = "30",
pages = "904–913",
journal = "Behavioral Ecology",
issn = "1045-2249",
publisher = "Oxford University Press",
number = "4",

}

Plastic expression of seminal fluid protein genes in a simultaneously hermaphroditic snail. / Nakadera, Yumi; Giannakara, Athina; Ramm, Steven A.

In: Behavioral Ecology, Vol. 30, No. 4, arz027, 07.2019, p. 904–913.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Plastic expression of seminal fluid protein genes in a simultaneously hermaphroditic snail

AU - Nakadera, Yumi

AU - Giannakara, Athina

AU - Ramm, Steven A

PY - 2019/7

Y1 - 2019/7

N2 - The eukaryotic RNA exosome is an essential, multi-subunit complex that catalyzes RNA turnover, maturation, and quality control processes. Its non-catalytic donut-shaped core includes 9 subunits that associate with the 3' to 5' exoribonucleases Rrp6, and Rrp44/Dis3, a subunit that also catalyzes endoribonuclease activity. Although recent structures and biochemical studies of RNA bound exosomes from S. cerevisiae revealed that the Exo9 central channel guides RNA to either Rrp6 or Rrp44 using partially overlapping and mutually exclusive paths, several issues related to RNA recruitment remain. Here, we identify activities for the highly basic Rrp6 C-terminal tail that we term the 'lasso' because it binds RNA and stimulates ribonuclease activities associated with Rrp44 and Rrp6 within the 11-subunit nuclear exosome. Stimulation is dependent on the Exo9 central channel, and the lasso contributes to degradation and processing activities of exosome substrates in vitro and in vivo. Finally, we present evidence that the Rrp6 lasso may be a conserved feature of the eukaryotic RNA exosome.

AB - The eukaryotic RNA exosome is an essential, multi-subunit complex that catalyzes RNA turnover, maturation, and quality control processes. Its non-catalytic donut-shaped core includes 9 subunits that associate with the 3' to 5' exoribonucleases Rrp6, and Rrp44/Dis3, a subunit that also catalyzes endoribonuclease activity. Although recent structures and biochemical studies of RNA bound exosomes from S. cerevisiae revealed that the Exo9 central channel guides RNA to either Rrp6 or Rrp44 using partially overlapping and mutually exclusive paths, several issues related to RNA recruitment remain. Here, we identify activities for the highly basic Rrp6 C-terminal tail that we term the 'lasso' because it binds RNA and stimulates ribonuclease activities associated with Rrp44 and Rrp6 within the 11-subunit nuclear exosome. Stimulation is dependent on the Exo9 central channel, and the lasso contributes to degradation and processing activities of exosome substrates in vitro and in vivo. Finally, we present evidence that the Rrp6 lasso may be a conserved feature of the eukaryotic RNA exosome.

UR - http://www.mendeley.com/research/plastic-expression-seminal-fluid-protein-genes-simultaneously-hermaphroditic-snail

U2 - 10.1093/beheco/arz027

DO - 10.1093/beheco/arz027

M3 - Article

VL - 30

SP - 904

EP - 913

JO - Behavioral Ecology

JF - Behavioral Ecology

SN - 1045-2249

IS - 4

M1 - arz027

ER -