Polarized type 1 cytokine response and cell-mediated immunity determine genetic resistance to mousepox

Geeta Chaudhri, Vijay Panchanathan, R Mark L Buller, Alfons J M van den Eertwegh, Eric H J H M Claassen, Jie Zhou, Rosalind de Chazal, Jon D Laman, Gunasegaran Karupiah

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Ectromelia virus (ECTV), a natural mouse pathogen and an orthopoxvirus, has been used to investigate the correlation between polarized type 1 or type 2 immune responses and resistance to disease in poxvirus infections by using well defined resistant and susceptible mouse strains. Our data show that distinct differences exist in the cytokine profiles expressed in resistant and susceptible mice infected with ECTV. Resistant C57BL/6 mice generate a type 1 cytokine response [IFN-gamma, IL-2, and tumor necrosis factor (TNF)], within the first few days of infection, which is associated with strong cytotoxic T lymphocyte response (CTL) and recovery from ECTV infection. Susceptible strains of mice (BALB/c and A/J) on the other hand generate a type 2 cytokine response (IL-4 but little or no IFN-gamma and IL-2), which is associated with a weak or an absent CTL response, resulting in uncontrolled virus replication and death. Although deletion of IL-4 function alone did not change the outcome of infection in susceptible mice, the loss of IFN-gamma function in resistant mice abrogated natural killer (NK) cell and CTL effector functions resulting in fulminant disease and 100% mortality. Therefore, a clear link exists between the early production of specific type 1 cytokines, in particular, IFN-gamma, the nature of the cellular immune response, and disease outcome in this virus model. This finding in the mousepox model raises the possibility that inappropriate cytokine responses may result in increased susceptibility to smallpox in humans.

    Original languageEnglish
    Pages (from-to)9057-62
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume101
    Issue number24
    DOIs
    Publication statusPublished - 15 Jun 2004

    Keywords

    • Animals
    • CD8-Positive T-Lymphocytes
    • Cell Line
    • Cytokines
    • Ectromelia virus
    • Ectromelia, Infectious
    • Female
    • Genetic Predisposition to Disease
    • Immunity, Cellular
    • Immunoglobulin G
    • Immunoglobulin M
    • Interferon-gamma
    • Killer Cells, Natural
    • Mice
    • Mice, Inbred BALB C
    • Mice, Inbred C57BL
    • Neutralization Tests
    • Spleen
    • T-Lymphocytes, Cytotoxic
    • Viral Load
    • Journal Article
    • Research Support, Non-U.S. Gov't
    • Research Support, U.S. Gov't, P.H.S.

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