Polymorphonuclear neutrophil integrity and functionality are preserved when exposed to saliva

P. Rijkschroeff; S.T.G. Gunput; A.J.M. Ligtenberg; E.C.I. Veerman; B.G. Loos; E.A. Nicu

doi:10.1016/j.archoralbio.2018.04.019

Polymorphonuclear neutrophil integrity and functionality are preserved when exposed to saliva

P. Rijkschroeff, S.T.G. Gunput, A.J.M. Ligtenberg, E.C.I. Veerman, B.G. Loos, E.A. Nicu

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Objective: Polymorphonuclear neutrophils (PMNs) are the most abundant innate immune cells and are also important effectors in the maintenance of oral health. However, little is known about the effects of saliva on the PMN. We therefore aimed to investigate the effect of saliva on the PMNs’ morphology and functioning.
Design: Effect of saliva on the membrane integrity of PMNs isolated from blood was evaluated with FACS using Annexin V (apoptosis marker) and propidum iodide (membrane integrity marker). The effect on cell morphology was examined using transmission electron imaging. Binding and phagocytosis of the oral bacterium Fusobacterium nucleatum by PMNs was analysed by FACS. Reactive oxygen species (ROS) production was measured with chemiluminescence.
Results: Incubation with saliva for 60 min had no detectable effects on the membrane integrity or the morphology of PMNs. In contrast, preincubation of F. nucleatum with saliva inhibited its subsequent interaction with PMNs, resulting in a diminished production of ROS.
Conclusions: Saliva does not impair the function of PMNs. However, interaction of salivary components with F. nucleatum may affect their recognition by PMNs resulting in a diminished functional response.

Original language	English
Pages (from-to)	68-74
Journal	Archives of Oral Biology
Volume	92
DOIs	https://doi.org/10.1016/j.archoralbio.2018.04.019
Publication status	Published - 2018

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Both the department of Periodontology and the department of Oral Biochemistry are supported by a grant from the University of Amsterdam for research into the focal point “Oral Infections and Inflammation”.

Funders	Funder number
Department of Periodontology
Universiteit van Amsterdam

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10.1016/j.archoralbio.2018.04.019

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title = "Polymorphonuclear neutrophil integrity and functionality are preserved when exposed to saliva",

abstract = "Objective: Polymorphonuclear neutrophils (PMNs) are the most abundant innate immune cells and are also important effectors in the maintenance of oral health. However, little is known about the effects of saliva on the PMN. We therefore aimed to investigate the effect of saliva on the PMNs{\textquoteright} morphology and functioning. Design: Effect of saliva on the membrane integrity of PMNs isolated from blood was evaluated with FACS using Annexin V (apoptosis marker) and propidum iodide (membrane integrity marker). The effect on cell morphology was examined using transmission electron imaging. Binding and phagocytosis of the oral bacterium Fusobacterium nucleatum by PMNs was analysed by FACS. Reactive oxygen species (ROS) production was measured with chemiluminescence. Results: Incubation with saliva for 60 min had no detectable effects on the membrane integrity or the morphology of PMNs. In contrast, preincubation of F. nucleatum with saliva inhibited its subsequent interaction with PMNs, resulting in a diminished production of ROS. Conclusions: Saliva does not impair the function of PMNs. However, interaction of salivary components with F. nucleatum may affect their recognition by PMNs resulting in a diminished functional response.",

author = "P. Rijkschroeff and S.T.G. Gunput and A.J.M. Ligtenberg and E.C.I. Veerman and B.G. Loos and E.A. Nicu",

year = "2018",

doi = "10.1016/j.archoralbio.2018.04.019",

language = "English",

volume = "92",

pages = "68--74",

journal = "Archives of Oral Biology",

issn = "0003-9969",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - Polymorphonuclear neutrophil integrity and functionality are preserved when exposed to saliva

AU - Rijkschroeff, P.

AU - Gunput, S.T.G.

AU - Ligtenberg, A.J.M.

AU - Veerman, E.C.I.

AU - Loos, B.G.

AU - Nicu, E.A.

PY - 2018

Y1 - 2018

N2 - Objective: Polymorphonuclear neutrophils (PMNs) are the most abundant innate immune cells and are also important effectors in the maintenance of oral health. However, little is known about the effects of saliva on the PMN. We therefore aimed to investigate the effect of saliva on the PMNs’ morphology and functioning. Design: Effect of saliva on the membrane integrity of PMNs isolated from blood was evaluated with FACS using Annexin V (apoptosis marker) and propidum iodide (membrane integrity marker). The effect on cell morphology was examined using transmission electron imaging. Binding and phagocytosis of the oral bacterium Fusobacterium nucleatum by PMNs was analysed by FACS. Reactive oxygen species (ROS) production was measured with chemiluminescence. Results: Incubation with saliva for 60 min had no detectable effects on the membrane integrity or the morphology of PMNs. In contrast, preincubation of F. nucleatum with saliva inhibited its subsequent interaction with PMNs, resulting in a diminished production of ROS. Conclusions: Saliva does not impair the function of PMNs. However, interaction of salivary components with F. nucleatum may affect their recognition by PMNs resulting in a diminished functional response.

AB - Objective: Polymorphonuclear neutrophils (PMNs) are the most abundant innate immune cells and are also important effectors in the maintenance of oral health. However, little is known about the effects of saliva on the PMN. We therefore aimed to investigate the effect of saliva on the PMNs’ morphology and functioning. Design: Effect of saliva on the membrane integrity of PMNs isolated from blood was evaluated with FACS using Annexin V (apoptosis marker) and propidum iodide (membrane integrity marker). The effect on cell morphology was examined using transmission electron imaging. Binding and phagocytosis of the oral bacterium Fusobacterium nucleatum by PMNs was analysed by FACS. Reactive oxygen species (ROS) production was measured with chemiluminescence. Results: Incubation with saliva for 60 min had no detectable effects on the membrane integrity or the morphology of PMNs. In contrast, preincubation of F. nucleatum with saliva inhibited its subsequent interaction with PMNs, resulting in a diminished production of ROS. Conclusions: Saliva does not impair the function of PMNs. However, interaction of salivary components with F. nucleatum may affect their recognition by PMNs resulting in a diminished functional response.

U2 - 10.1016/j.archoralbio.2018.04.019

DO - 10.1016/j.archoralbio.2018.04.019

M3 - Article

C2 - 29763780

SN - 0003-9969

VL - 92

SP - 68

EP - 74

JO - Archives of Oral Biology

JF - Archives of Oral Biology

ER -

Polymorphonuclear neutrophil integrity and functionality are preserved when exposed to saliva

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