Population genetic structure and cladistic analysis of Trypanosoma brucei isolates

Eddy Chukwura Agbo, Peter-Henning Clausen, Philippe Büscher, Phelix A O Majiwa, Eric Claassen, Marinus F W te Pas

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then performed a cladistic analysis to trace relationships and evolution, using stocks and clones recovered from geographically and temporally matched hosts, including inter-strain comparisons with T. b. gambiense stocks and clones. Our results show that while there was close genetic relatedness among parasite populations from the same geographical region, micro-heterogeneities exist between different stocks. Data are presented that indicate that not every human sleeping sickness focus may be associated with a particular human-infective trypanosome strain responsible for long-term stability of the reference focus. We provide evidence of genetic sub-structuring among type 1 T. b. gambiense stocks, which has potentially important implications for molecular epidemiology of T. brucei.

    Original languageEnglish
    Pages (from-to)165-74
    Number of pages10
    JournalInfection, Genetics and Evolution
    Volume3
    Issue number3
    DOIs
    Publication statusPublished - Sep 2003

    Keywords

    • Animals
    • Biological Evolution
    • Endemic Diseases
    • Genetics, Population
    • Host-Parasite Interactions
    • Humans
    • Models, Genetic
    • Phylogeny
    • Polymorphism, Genetic
    • Trypanosoma brucei brucei
    • Trypanosomiasis, African
    • Uganda
    • Journal Article
    • Research Support, Non-U.S. Gov't

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