Porous multilayer-coated AFM tips for dip-pen nanolithography of proteins

Chien-Ching Wu, Huaping Xu, Cees Otto, David N Reinhoudt, Rob G H Lammertink, Jurriaan Huskens, Vinod Subramaniam, Aldrik H Velders

    Research output: Contribution to JournalArticleAcademicpeer-review


    A simple and novel method for fabricating nanoporous-structure-coated silicon nitride tips for dip-pen nanolithography (DPN) by using the layer-by-layer (LbL) technique has been developed. The pore sizes can be adjusted by treating the LbL films coated onto the amino-terminated self-assembled monolayer (NH(2)-SAM)-functionalized AFM tip surface with a base solution for different periods of time. This hydrophilic porous material can absorb biomolecules easily and also provides a larger-volume ink reservoir compared with a bare silicon nitride tip. Proof-of-concept of the porous AFM tip is demonstrated by using fluorescent proteins as ink molecules to fabricate protein patterns at the micrometer and submicrometer length scales.

    Original languageEnglish
    Pages (from-to)7526-7
    Number of pages2
    JournalJournal of the American Chemical Society
    Issue number22
    Publication statusPublished - 10 Jun 2009


    • Acrylates
    • Green Fluorescent Proteins
    • Microscopy, Atomic Force
    • Nanotechnology
    • Polyvinyls
    • Proteins
    • Silicon Compounds
    • Journal Article
    • Research Support, Non-U.S. Gov't


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