Porous multilayer-coated AFM tips for dip-pen nanolithography of proteins

Chien-Ching Wu; Huaping Xu; Cees Otto; David N Reinhoudt; Rob G H Lammertink; Jurriaan Huskens; Vinod Subramaniam; Aldrik H Velders

doi:10.1021/ja901756a

Porous multilayer-coated AFM tips for dip-pen nanolithography of proteins

Chien-Ching Wu, Huaping Xu, Cees Otto, David N Reinhoudt, Rob G H Lammertink, Jurriaan Huskens, Vinod Subramaniam, Aldrik H Velders

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A simple and novel method for fabricating nanoporous-structure-coated silicon nitride tips for dip-pen nanolithography (DPN) by using the layer-by-layer (LbL) technique has been developed. The pore sizes can be adjusted by treating the LbL films coated onto the amino-terminated self-assembled monolayer (NH(2)-SAM)-functionalized AFM tip surface with a base solution for different periods of time. This hydrophilic porous material can absorb biomolecules easily and also provides a larger-volume ink reservoir compared with a bare silicon nitride tip. Proof-of-concept of the porous AFM tip is demonstrated by using fluorescent proteins as ink molecules to fabricate protein patterns at the micrometer and submicrometer length scales.

Original language	English
Pages (from-to)	7526-7
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	131
Issue number	22
DOIs	https://doi.org/10.1021/ja901756a
Publication status	Published - 10 Jun 2009

Keywords

Acrylates
Green Fluorescent Proteins
Microscopy, Atomic Force
Nanotechnology
Polyvinyls
Proteins
Silicon Compounds
Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/ja901756a

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title = "Porous multilayer-coated AFM tips for dip-pen nanolithography of proteins",

abstract = "A simple and novel method for fabricating nanoporous-structure-coated silicon nitride tips for dip-pen nanolithography (DPN) by using the layer-by-layer (LbL) technique has been developed. The pore sizes can be adjusted by treating the LbL films coated onto the amino-terminated self-assembled monolayer (NH(2)-SAM)-functionalized AFM tip surface with a base solution for different periods of time. This hydrophilic porous material can absorb biomolecules easily and also provides a larger-volume ink reservoir compared with a bare silicon nitride tip. Proof-of-concept of the porous AFM tip is demonstrated by using fluorescent proteins as ink molecules to fabricate protein patterns at the micrometer and submicrometer length scales.",

keywords = "Acrylates, Green Fluorescent Proteins, Microscopy, Atomic Force, Nanotechnology, Polyvinyls, Proteins, Silicon Compounds, Journal Article, Research Support, Non-U.S. Gov't",

author = "Chien-Ching Wu and Huaping Xu and Cees Otto and Reinhoudt, \{David N\} and Lammertink, \{Rob G H\} and Jurriaan Huskens and Vinod Subramaniam and Velders, \{Aldrik H\}",

year = "2009",

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doi = "10.1021/ja901756a",

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AU - Wu, Chien-Ching

AU - Xu, Huaping

AU - Otto, Cees

AU - Reinhoudt, David N

AU - Lammertink, Rob G H

AU - Huskens, Jurriaan

AU - Subramaniam, Vinod

AU - Velders, Aldrik H

PY - 2009/6/10

Y1 - 2009/6/10

N2 - A simple and novel method for fabricating nanoporous-structure-coated silicon nitride tips for dip-pen nanolithography (DPN) by using the layer-by-layer (LbL) technique has been developed. The pore sizes can be adjusted by treating the LbL films coated onto the amino-terminated self-assembled monolayer (NH(2)-SAM)-functionalized AFM tip surface with a base solution for different periods of time. This hydrophilic porous material can absorb biomolecules easily and also provides a larger-volume ink reservoir compared with a bare silicon nitride tip. Proof-of-concept of the porous AFM tip is demonstrated by using fluorescent proteins as ink molecules to fabricate protein patterns at the micrometer and submicrometer length scales.

AB - A simple and novel method for fabricating nanoporous-structure-coated silicon nitride tips for dip-pen nanolithography (DPN) by using the layer-by-layer (LbL) technique has been developed. The pore sizes can be adjusted by treating the LbL films coated onto the amino-terminated self-assembled monolayer (NH(2)-SAM)-functionalized AFM tip surface with a base solution for different periods of time. This hydrophilic porous material can absorb biomolecules easily and also provides a larger-volume ink reservoir compared with a bare silicon nitride tip. Proof-of-concept of the porous AFM tip is demonstrated by using fluorescent proteins as ink molecules to fabricate protein patterns at the micrometer and submicrometer length scales.

KW - Acrylates

KW - Green Fluorescent Proteins

KW - Microscopy, Atomic Force

KW - Nanotechnology

KW - Polyvinyls

KW - Proteins

KW - Silicon Compounds

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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ER -

Porous multilayer-coated AFM tips for dip-pen nanolithography of proteins

Abstract

Keywords

UN SDGs

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