Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Forster Resonance Energy Transfer (FRET) studies.

M.A. Hink; N.V. Visser; J.W. Borst; A. Hoek; A.J.W.G. Visser

doi:10.1023/A:1022947411788

Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Forster Resonance Energy Transfer (FRET) studies.

M.A. Hink, N.V. Visser, J.W. Borst, A. Hoek, A.J.W.G. Visser

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Corrected fluorescence excitation and emission spectra have been obtained from several enhanced variants of the green fluorescent protein (EGFP) isolated from the jellyfish Aequorea victoria, blue fluorescence protein (EBFP), cyan fluorescent protein (ECFP), EGFP and yellow fluorescent protein (EYFP-citrine) and from the red fluorescent protein (DsRed) isolated from the coral species Discosoma. The spectra are stored in a database. This report describes how the spectra can be used as templates to derive the critical transfer distance for any pair of fluorescent proteins.

Original language	English
Pages (from-to)	185-188
Number of pages	3
Journal	Journal of Fluorescence
Volume	13
DOIs	https://doi.org/10.1023/A:1022947411788
Publication status	Published - 2003

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title = "Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Forster Resonance Energy Transfer (FRET) studies.",

abstract = "Corrected fluorescence excitation and emission spectra have been obtained from several enhanced variants of the green fluorescent protein (EGFP) isolated from the jellyfish Aequorea victoria, blue fluorescence protein (EBFP), cyan fluorescent protein (ECFP), EGFP and yellow fluorescent protein (EYFP-citrine) and from the red fluorescent protein (DsRed) isolated from the coral species Discosoma. The spectra are stored in a database. This report describes how the spectra can be used as templates to derive the critical transfer distance for any pair of fluorescent proteins.",

author = "M.A. Hink and N.V. Visser and J.W. Borst and A. Hoek and A.J.W.G. Visser",

year = "2003",

doi = "10.1023/A:1022947411788",

language = "English",

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pages = "185--188",

journal = "Journal of Fluorescence",

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T1 - Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Forster Resonance Energy Transfer (FRET) studies.

AU - Hink, M.A.

AU - Visser, N.V.

AU - Borst, J.W.

AU - Hoek, A.

AU - Visser, A.J.W.G.

PY - 2003

Y1 - 2003

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AB - Corrected fluorescence excitation and emission spectra have been obtained from several enhanced variants of the green fluorescent protein (EGFP) isolated from the jellyfish Aequorea victoria, blue fluorescence protein (EBFP), cyan fluorescent protein (ECFP), EGFP and yellow fluorescent protein (EYFP-citrine) and from the red fluorescent protein (DsRed) isolated from the coral species Discosoma. The spectra are stored in a database. This report describes how the spectra can be used as templates to derive the critical transfer distance for any pair of fluorescent proteins.

U2 - 10.1023/A:1022947411788

DO - 10.1023/A:1022947411788

M3 - Article

SN - 1053-0509

VL - 13

SP - 185

EP - 188

JO - Journal of Fluorescence

JF - Journal of Fluorescence

ER -

Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Forster Resonance Energy Transfer (FRET) studies.

Abstract

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