TY - JOUR
T1 - Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Forster Resonance Energy Transfer (FRET) studies.
AU - Hink, M.A.
AU - Visser, N.V.
AU - Borst, J.W.
AU - Hoek, A.
AU - Visser, A.J.W.G.
PY - 2003
Y1 - 2003
N2 - Corrected fluorescence excitation and emission spectra have been obtained from several enhanced variants of the green fluorescent protein (EGFP) isolated from the jellyfish Aequorea victoria, blue fluorescence protein (EBFP), cyan fluorescent protein (ECFP), EGFP and yellow fluorescent protein (EYFP-citrine) and from the red fluorescent protein (DsRed) isolated from the coral species Discosoma. The spectra are stored in a database. This report describes how the spectra can be used as templates to derive the critical transfer distance for any pair of fluorescent proteins.
AB - Corrected fluorescence excitation and emission spectra have been obtained from several enhanced variants of the green fluorescent protein (EGFP) isolated from the jellyfish Aequorea victoria, blue fluorescence protein (EBFP), cyan fluorescent protein (ECFP), EGFP and yellow fluorescent protein (EYFP-citrine) and from the red fluorescent protein (DsRed) isolated from the coral species Discosoma. The spectra are stored in a database. This report describes how the spectra can be used as templates to derive the critical transfer distance for any pair of fluorescent proteins.
UR - https://www.scopus.com/pages/publications/0347720886
UR - https://www.scopus.com/inward/citedby.url?scp=0347720886&partnerID=8YFLogxK
U2 - 10.1023/A:1022947411788
DO - 10.1023/A:1022947411788
M3 - Article
SN - 1053-0509
VL - 13
SP - 185
EP - 188
JO - Journal of Fluorescence
JF - Journal of Fluorescence
ER -
