Pressure-induced shifts in trophic linkages in a simplified aquatic food web

Maarten Schrama*, S. Henrik Barmentlo, Ellard R. Hunting, Richard S.P. van Logtestijn, Martina G. Vijver, Peter M. van Bodegom

*Corresponding author for this work

    Research output: Contribution to JournalArticleAcademicpeer-review


    It is essential to understand effects of existing and emerging anthropogenic stressors on the structure of aquatic food webs in more natural settings, to obtain realistic predictions on how they can affect major ecosystem properties and functioning. We therefore examined whether (1) realistic concentrations of key agricultural pesticides and nutrients induce shifts in trophic linkages (2) observed changes in trophic linkages are qualitatively different between the green (algal-based) and brown (detritus-based) part of the food web. To this end, we exposed a simplified, yet realistic freshwater invertebrate community to environmentally relevant concentrations of three anthropogenic pressures (eutrophication; the herbicide terbuthylazine; and the insecticide imidacloprid) in a full factorial mesocosm design. Trophic linkages and the changes therein were assessed measuring stable isotopes of natural carbon and nitrogen. Results show that the green and brown part of the food web react qualitatively different to interacting pressures. Whereas, herbivorous species react mainly to the nutrients and herbicides and the synergistic interaction between these, species in the detritivore part of the food web were affected by insecticide applications and interactions with nutrients. These results suggest that agricultural pressures can induce shifts in trophic linkages, but that they can have contrasting effects on the different parts of the food web. Such antagonistic and synergistic interactions can provide powerful explanations for observed responses of ecosystems to interacting stressors. These findings may have important implications for our understanding on interactions of agricultural stressors and their propagation in aquatic food webs.

    Original languageEnglish
    Article number75
    JournalFrontiers in Environmental Science
    Issue numberDEC
    Publication statusPublished - 4 Dec 2017


    We are grateful to the Hortus Botanicus in Leiden for providing the space and help to run this experiment, most notably Prof Paul Keßler and Theo Houthoff. We would like to thank Rody Blom, Arjen van Putten, and Erik Gertenaar for practical assistance and colleagues at the CML for their help with collecting the data. SB, EH, and MV were funded by NWO-ASPASIA and NWO-VIDI 864.13.010 granted to MV

    FundersFunder number
    Nederlandse Organisatie voor Wetenschappelijk Onderzoek864.13.010


      • Anthropogenic pressures
      • Brown web
      • Green web
      • Imidacloprid
      • Interaction web
      • Multiple stressors
      • Stable isotopes
      • Terbuthylazine


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