Primary electron transfer kinetics in membrane-bound Rhodobacter sphaeroides reaction centers: A global and target analysis.

I.H.M. van Stokkum, L.M.P. Beekman, M.R. Jones, M.E. van Brederode, R. van Grondelle

Research output: Contribution to JournalArticleAcademicpeer-review

166 Downloads (Pure)

Abstract

Absorbance difference kinetics were measured on quinone-reduced membrane-bound wild type. Rhodobacter sphaeroides reaction centers in the wavelength region from 690 to 1060 nm using 800 nm excitation. Global analysis of the data revealed five lifetimes of 0.18, 1.9, 5.1, and 22 ps and a long-lived component for the processes that underlie the spectral evolution of the system. The 0.18 ps component was ascribed to energy transfer from the excited state of the accessory bacteriochlorophyll (B*) to the primary donor (P*). The 1.9 ps component was associated with a state involving a BChl anion absorbing in the 1020 nm region. This led to the conclusion that primary electron transfer is best described by a model in which the electron is passed from P* to the acceptor bacteriopheophytin (H(L)) via the monomeric bacteriochlorophyll (B(L)), with the formation of the radical pair state P
Original languageEnglish
Pages (from-to)11360-11368
JournalBiochemistry
Volume36
DOIs
Publication statusPublished - 1997

Fingerprint

Dive into the research topics of 'Primary electron transfer kinetics in membrane-bound Rhodobacter sphaeroides reaction centers: A global and target analysis.'. Together they form a unique fingerprint.

Cite this