Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry

M.T.A. Alexandre; T. Domratcheva; C. Bonetti; L. van Wilderen; M.L. Groot; R. van Grondelle; K.J. Hellingwerf; J.T.M. Kennis

doi:10.1016/j.bpj.2009.01.066

Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry

M.T.A. Alexandre, T. Domratcheva, C. Bonetti, L. van Wilderen, M.L. Groot, R. van Grondelle, K.J. Hellingwerf, J.T.M. Kennis

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. Here, the primary reactions of Avena sativa phototropin 1 LOV2 (AsLOV2) were studied using ultrafast mid-infrared spectroscopy and quantum chemistry. The singlet excited state (S1) evolves into the triplet state (T1) with a lifetime of 1.5 ns at a yield of ̃50%. The infrared signature of S1 is characterized by absorption bands at 1657 cm

Original language	English
Pages (from-to)	227-237
Journal	Biophysical Journal
Volume	97
Issue number	1
DOIs	https://doi.org/10.1016/j.bpj.2009.01.066
Publication status	Published - 2009

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Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry

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10.1016/j.bpj.2009.01.066

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title = "Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry",

abstract = "Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. Here, the primary reactions of Avena sativa phototropin 1 LOV2 (AsLOV2) were studied using ultrafast mid-infrared spectroscopy and quantum chemistry. The singlet excited state (S1) evolves into the triplet state (T1) with a lifetime of 1.5 ns at a yield of{\~ }50%. The infrared signature of S1 is characterized by absorption bands at 1657 cm",

author = "M.T.A. Alexandre and T. Domratcheva and C. Bonetti and {van Wilderen}, L. and M.L. Groot and {van Grondelle}, R. and K.J. Hellingwerf and J.T.M. Kennis",

note = "Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry",

year = "2009",

doi = "10.1016/j.bpj.2009.01.066",

language = "English",

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pages = "227--237",

journal = "Biophysical Journal",

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TY - JOUR

T1 - Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry

AU - Alexandre, M.T.A.

AU - Domratcheva, T.

AU - Bonetti, C.

AU - van Wilderen, L.

AU - Groot, M.L.

AU - van Grondelle, R.

AU - Hellingwerf, K.J.

AU - Kennis, J.T.M.

N1 - Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry

PY - 2009

Y1 - 2009

N2 - Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. Here, the primary reactions of Avena sativa phototropin 1 LOV2 (AsLOV2) were studied using ultrafast mid-infrared spectroscopy and quantum chemistry. The singlet excited state (S1) evolves into the triplet state (T1) with a lifetime of 1.5 ns at a yield of ̃50%. The infrared signature of S1 is characterized by absorption bands at 1657 cm

AB - Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. Here, the primary reactions of Avena sativa phototropin 1 LOV2 (AsLOV2) were studied using ultrafast mid-infrared spectroscopy and quantum chemistry. The singlet excited state (S1) evolves into the triplet state (T1) with a lifetime of 1.5 ns at a yield of ̃50%. The infrared signature of S1 is characterized by absorption bands at 1657 cm

U2 - 10.1016/j.bpj.2009.01.066

DO - 10.1016/j.bpj.2009.01.066

M3 - Article

SN - 0006-3495

VL - 97

SP - 227

EP - 237

JO - Biophysical Journal

JF - Biophysical Journal

IS - 1

ER -

Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry

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