Probing cellular mechanics with acoustic force spectroscopy

Raya Sorkin, Giulia Bergamaschi, Douwe Kamsma, Guy Brand, Elya Dekel, Yifat Ofir-Birin, Ariel Rudik, Marta Gironella, Felix Ritort, Neta Regev-Rudzki, Wouter H. Roos, Gijs J.L. Wuite

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability of red blood cells (RBCs) is key to their function and is dramatically altered in the time course of diseases such as anemia and malaria. Due to the physiological importance of cell mechanics, many methods for cell mechanical probing have been developed. While single-cell methods provide very valuable information, they are often technically challenging and lack the high data throughput needed to distinguish differences in heterogeneous populations, while fluid-flow high-throughput methods miss the accuracy to detect subtle differences. Here we present a new method for multiplexed single-cell mechanical probing using acoustic force spectroscopy (AFS). We demonstrate that mechanical differences induced by chemical treatments of known effect can be measured and quantified. Furthermore, we explore the effect of extracellular vesicles (EVs) uptake on RBC mechanics and demonstrate that EVs uptake increases RBC deformability. Our findings demonstrate the ability of AFS to manipulate cells with high stability and precision and pave the way to further new insights into cellular mechanics and mechanobiology in health and disease, as well as potential biomedical applications.

Original languageEnglish
Pages (from-to)2005-2011
Number of pages7
JournalMolecular Biology of the Cell
Volume29
Issue number16
DOIs
Publication statusPublished - 8 Aug 2018

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Mechanics
Acoustics
Spectrum Analysis
Erythrocytes
Biophysics
Malaria
Anemia
Pathology
Health
Population

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Sorkin, Raya ; Bergamaschi, Giulia ; Kamsma, Douwe ; Brand, Guy ; Dekel, Elya ; Ofir-Birin, Yifat ; Rudik, Ariel ; Gironella, Marta ; Ritort, Felix ; Regev-Rudzki, Neta ; Roos, Wouter H. ; Wuite, Gijs J.L. / Probing cellular mechanics with acoustic force spectroscopy. In: Molecular Biology of the Cell. 2018 ; Vol. 29, No. 16. pp. 2005-2011.
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Sorkin, R, Bergamaschi, G, Kamsma, D, Brand, G, Dekel, E, Ofir-Birin, Y, Rudik, A, Gironella, M, Ritort, F, Regev-Rudzki, N, Roos, WH & Wuite, GJL 2018, 'Probing cellular mechanics with acoustic force spectroscopy' Molecular Biology of the Cell, vol. 29, no. 16, pp. 2005-2011. https://doi.org/10.1091/mbc.E18-03-0154

Probing cellular mechanics with acoustic force spectroscopy. / Sorkin, Raya; Bergamaschi, Giulia; Kamsma, Douwe; Brand, Guy; Dekel, Elya; Ofir-Birin, Yifat; Rudik, Ariel; Gironella, Marta; Ritort, Felix; Regev-Rudzki, Neta; Roos, Wouter H.; Wuite, Gijs J.L.

In: Molecular Biology of the Cell, Vol. 29, No. 16, 08.08.2018, p. 2005-2011.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Bergamaschi, Giulia

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AU - Ofir-Birin, Yifat

AU - Rudik, Ariel

AU - Gironella, Marta

AU - Ritort, Felix

AU - Regev-Rudzki, Neta

AU - Roos, Wouter H.

AU - Wuite, Gijs J.L.

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