Probing the formation of isolated cyclo-FF peptide clusters by far-infrared action spectroscopy

Sjors Bakels, Iuliia Stroganova, Anouk M. Rijs*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Small cyclic peptides containing phenylalanine residues are prone to aggregate in the gas phase into highly hydrophobic chains. A combination of laser desorption, mass spectrometry and conformational selective IR-UV action spectroscopy allows us to obtain detailed structural insights into the formation processes of the cyclic l-phenylalanyl-l-phenylalanine dipeptide (named cyclo-FF) aggregates. The rigid properties of cyclo-FF result in highly resolved IR spectra for the smaller clusters (n ≤ 3) and corresponding conformational assignments. For the higher order clusters (n > 3) the spectra are less resolved, however the observed ratios, peak positions and trends in IR shifts are key to make predictions on their structural details. Whereas the mid-IR spectral region between 1000-1800 cm-1 turns out to be undiagnostic for these small aggregates and the 3 μm region only for specific calculated structures, the far-IR contains valuable information that allows for clear assignments.

Original languageEnglish
Pages (from-to)20945-20956
Number of pages12
JournalPhysical Chemistry Chemical Physics
Volume23
Issue number37
Early online date15 Sept 2021
DOIs
Publication statusPublished - 7 Oct 2021

Bibliographical note

Funding Information:
We gratefully acknowledge the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) for the support of the FELIX Laboratory. We thank the FELIX team for their experimental support and helpful discussions. This work was sponsored by NWO Exact and Natural Sciences for the use of supercomputer facilities (Grant no. 2019.062) at the SurfSARA Cartesius cluster. Furthermore, Alexander Lemmens is particularly acknowledged for his help on the OPO measurements, and helpful discussions in general.

Publisher Copyright:
© 2021 the Owner Societies.

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Fingerprint

Dive into the research topics of 'Probing the formation of isolated cyclo-FF peptide clusters by far-infrared action spectroscopy'. Together they form a unique fingerprint.

Cite this