@article{00014e4e935b446c84e9b2427ded8cd3,
title = "Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling",
abstract = "Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interactions due to its ability to image single molecules at the nanoscale. However, the use of AFM in force spectroscopy to study DNA-protein interactions has been limited. Here we developed a high throughput, AFM based, pulling assay to measure the strength and kinetics of protein bridging of DNA molecules. As a model system, we investigated the interactions between DNA and the Histone-like Nucleoid-Structuring protein (H-NS). We confirmed that H-NS both changes DNA rigidity and forms bridges between DNA molecules. This straightforward methodology provides a high-throughput approach with single-molecule resolution which is widely applicable to study cross-substrate interactions such as DNA-bridging proteins.",
author = "Yan Liang and {van der Valk}, {Ramon A.} and Dame, {Remus T.} and Roos, {Wouter H.} and Wuite, {Gijs J. L.}",
year = "2017",
month = nov,
day = "10",
doi = "10.1038/s41598-017-15477-4",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Research",
number = "1",
}