Protective effect of N-acetylcysteine on the toxicity of silver nanoparticles: Bioavailability and toxicokinetics in Enchytraeus crypticus

Monique Culturato Padilha Mendonça*, Marcelo Bispo de Jesus, Cornelis A.M. van Gestel

*Corresponding author for this work

    Research output: Contribution to JournalArticleAcademicpeer-review

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    Abstract

    We previously demonstrated that N-acetylcysteine (NAC) could reduce the toxicity of silver (Ag) materials (nanoparticles (NPs) and Ag nitrate) to the soil invertebrate Enchytraeus crypticus (Oligochaeta). It remains however, unclear whether the antitoxic mechanism of NAC was caused by NAC-Ag binding in the soil or inside the organisms. This study aimed at determining the bioavailability of Ag in the soil in a 21-day toxicity test as well as the Ag uptake and elimination kinetics in E. crypticus exposed to AgNPs in LUFA 2.2 standard soil amended with low (100 mg/kg dry soil) and high (600 mg/kg dry soil) NAC concentrations. The addition of NAC to the soil alleviated the toxicity of AgNPs by decreasing the internal Ag concentration of E. crypticus in a dose-dependent manner. Indeed, NAC reduced the binding of Ag to the soil, which probably was due to the formation of soluble but biologically unavailable Ag-cysteine complexes. The reduced Ag uptake in the enchytraeids was explained from an increased elimination at high NAC levels. These findings reinforce the view that metal complexing-compounds like NAC play a key role in the modulation of AgNP toxicity and bioavailability in terrestrial environments. Further, it may inform on the potential of NAC as a remediation solution for Ag or other metal-contaminated soils.

    Original languageEnglish
    Article number136797
    Pages (from-to)1-8
    Number of pages8
    JournalScience of the Total Environment
    Volume715
    Early online date18 Jan 2020
    DOIs
    Publication statusPublished - 1 May 2020

    Funding

    We gratefully thank the EU H2020 project NanoFASE (Nanomaterial Fate and Speciation in the Environment; grant no. 646002) and Applied Nanoparticles SL for providing the AgNPs, and to Rudo A. Verweij for his technical support in the experimental work. This study was financially supported by São Paulo Research Foundation (FAPESP) (grant # 2018/24658-9 and # 2016/03765-6 ).

    FundersFunder number
    EU H2020
    Speciation in the Environment646002
    Fundação de Amparo à Pesquisa do Estado de São Paulo2016/03765-6, 2018/24658-9

      Keywords

      • Bioavailability
      • Metallic nanoparticles
      • Nanoecotoxicology
      • Thiol compounds

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