Protein mapping of peanut extract with capillary electrophoresis

Loïc Villemet, Aurélien Cuchet, Christophe Desvignes, Cari E Sänger-van de Griend

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Protein separation can be achieved with different modes of capillary electrophoresis, such as with capillary gel electroporesis (CGE) or with capillary zone electrophoresis (CZE). CZE protein mapping of peanut extract was approached in four different ways, combining neutral-coated or multilayer-coated capillaries with pHs well over or under the isoelectric point range of the proteins of interest. At acidic pHs, the mobility ranges of the major peanut allergens Ara h1, Ara h2, Ara h3, and Ara h6 were identified. Although the pH is a major factor in CZE separation, buffers with different compositions but with the same pH and ionic strength showed significantly different resolutions. Different components of the electrolyte were studied in a multifactorial design of experiment. CE-SDS and CZE proved to be suitable for protein mapping and we were able to distinguish different batches of peanut extract and burned peanut extract.

Original languageEnglish
JournalElectrophoresis
DOIs
Publication statusE-pub ahead of print - 10 May 2021
Externally publishedYes

Bibliographical note

© 2021 The Authors. Electrophoresis published by Wiley-VCH GmbH.

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