Proton transfer events in GFP

M. Di Donato; L.J.G.W. van Wilderen; I.H.M. van Stokkum; T.A. Cohen Stuart; J.T.M. Kennis; K.J. Hellingwerf; R. van Grondelle; M.L. Groot

doi:10.1039/c1cp20387h

Proton transfer events in GFP

M. Di Donato, L.J.G.W. van Wilderen, I.H.M. van Stokkum, T.A. Cohen Stuart, J.T.M. Kennis, K.J. Hellingwerf, R. van Grondelle, M.L. Groot

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Proton transfer is one of the most important elementary processes in biology. Green fluorescent protein (GFP) serves as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. Illumination initiates proton transfer through a 'proton-wire', formed by the chromophore (the proton donor), water molecule W22, Ser205 and Glu222 (the acceptor), on a picosecond time scale. To obtain a more refined view of this process, we have used a combined approach of time resolved mid-infrared spectroscopy and visible pump-dump-probe spectroscopy to resolve with atomic resolution how and how fast protons move through this wire. Our results indicate that absorption of light by GFP induces in 3 ps (10 ps in D

Original language	English
Pages (from-to)	16295-16305
Journal	Physical Chemistry Chemical Physics - PCCP
Volume	13
Issue number	36
DOIs	https://doi.org/10.1039/c1cp20387h
Publication status	Published - 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1039/c1cp20387h

Persistent URL (handle)

Copy link address

Cite this

@article{bedf9a8d1e7d400f803cd39249100539,

title = "Proton transfer events in GFP",

abstract = "Proton transfer is one of the most important elementary processes in biology. Green fluorescent protein (GFP) serves as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. Illumination initiates proton transfer through a 'proton-wire', formed by the chromophore (the proton donor), water molecule W22, Ser205 and Glu222 (the acceptor), on a picosecond time scale. To obtain a more refined view of this process, we have used a combined approach of time resolved mid-infrared spectroscopy and visible pump-dump-probe spectroscopy to resolve with atomic resolution how and how fast protons move through this wire. Our results indicate that absorption of light by GFP induces in 3 ps (10 ps in D",

author = "{Di Donato}, M. and {van Wilderen}, L.J.G.W. and {van Stokkum}, I.H.M. and {Cohen Stuart}, T.A. and J.T.M. Kennis and K.J. Hellingwerf and {van Grondelle}, R. and M.L. Groot",

year = "2011",

doi = "10.1039/c1cp20387h",

language = "English",

volume = "13",

pages = "16295--16305",

journal = "Physical Chemistry Chemical Physics - PCCP",

issn = "1463-9076",

publisher = "The Royal Society of Chemistry",

number = "36",

}

TY - JOUR

T1 - Proton transfer events in GFP

AU - Di Donato, M.

AU - van Wilderen, L.J.G.W.

AU - van Stokkum, I.H.M.

AU - Cohen Stuart, T.A.

AU - Kennis, J.T.M.

AU - Hellingwerf, K.J.

AU - van Grondelle, R.

AU - Groot, M.L.

PY - 2011

Y1 - 2011

N2 - Proton transfer is one of the most important elementary processes in biology. Green fluorescent protein (GFP) serves as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. Illumination initiates proton transfer through a 'proton-wire', formed by the chromophore (the proton donor), water molecule W22, Ser205 and Glu222 (the acceptor), on a picosecond time scale. To obtain a more refined view of this process, we have used a combined approach of time resolved mid-infrared spectroscopy and visible pump-dump-probe spectroscopy to resolve with atomic resolution how and how fast protons move through this wire. Our results indicate that absorption of light by GFP induces in 3 ps (10 ps in D

AB - Proton transfer is one of the most important elementary processes in biology. Green fluorescent protein (GFP) serves as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. Illumination initiates proton transfer through a 'proton-wire', formed by the chromophore (the proton donor), water molecule W22, Ser205 and Glu222 (the acceptor), on a picosecond time scale. To obtain a more refined view of this process, we have used a combined approach of time resolved mid-infrared spectroscopy and visible pump-dump-probe spectroscopy to resolve with atomic resolution how and how fast protons move through this wire. Our results indicate that absorption of light by GFP induces in 3 ps (10 ps in D

U2 - 10.1039/c1cp20387h

DO - 10.1039/c1cp20387h

M3 - Article

SN - 1463-9076

VL - 13

SP - 16295

EP - 16305

JO - Physical Chemistry Chemical Physics - PCCP

JF - Physical Chemistry Chemical Physics - PCCP

IS - 36

ER -

Proton transfer events in GFP

Abstract

UN SDGs

Access to Document

Persistent URL (handle)

Fingerprint

Cite this