Proton transfer events in GFP

M. Di Donato, L.J.G.W. van Wilderen, I.H.M. van Stokkum, T.A. Cohen Stuart, J.T.M. Kennis, K.J. Hellingwerf, R. van Grondelle, M.L. Groot

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Proton transfer is one of the most important elementary processes in biology. Green fluorescent protein (GFP) serves as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. Illumination initiates proton transfer through a 'proton-wire', formed by the chromophore (the proton donor), water molecule W22, Ser205 and Glu222 (the acceptor), on a picosecond time scale. To obtain a more refined view of this process, we have used a combined approach of time resolved mid-infrared spectroscopy and visible pump-dump-probe spectroscopy to resolve with atomic resolution how and how fast protons move through this wire. Our results indicate that absorption of light by GFP induces in 3 ps (10 ps in D
Original languageEnglish
Pages (from-to)16295-16305
JournalPhysical Chemistry Chemical Physics - PCCP
Volume13
Issue number36
DOIs
Publication statusPublished - 2011

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