PSB33 sustains photosystem II D1 protein under fluctuating light conditions

Rikard Fristedt, Andrea Trotta, Marjaana Suorsa, Anders K. Nilsson, Roberta Croce, Eva Mari Aro, Björn Lundin

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

On Earth, solar irradiance varies as the sun rises and sets over the horizon, and sunlight is thus in constant fluctuation, following a slow dark–low–high–low–dark curve. Optimal plant growth and development are dependent on the capacity of plants to acclimate and regulate photosynthesis in response to these changes of light. Little is known of regulative processes for photosynthesis during nocturnal events. The nucleus-encoded plant lineage-specific protein PSB33 has been described as stabilizing the photosystem II complex, especially under light stress conditions, and plants lacking PSB33 have a dysfunctional state transition. To clarify the localization and function of this protein, we used phenomic, biochemical and proteomics approaches in the model plant Arabidopsis. We report that PSB33 is predominantly located in non-appressed thylakoid regions and dynamically associates with a thylakoid protein complex in a light-dependent manner. Moreover, plants lacking PSB33 show an accelerated D1 protein degradation in nocturnal periods, and show severely stunted growth when challenged with fluctuating light. We further show that the function of PSB33 precedes the STN7 kinase to regulate or balance the excitation energy of photosystems I and II in fluctuating light conditions.

Original languageEnglish
Pages (from-to)4281-4293
Number of pages13
JournalJournal of Experimental Botany
Volume68
Issue number15
DOIs
Publication statusPublished - 10 Jul 2017

Fingerprint

D1 protein
Photosystem II Protein Complex
photosystem II
Light
Thylakoids
Photosynthesis
Proteins
thylakoids
solar radiation
photosynthesis
Growth Disorders
Photosystem I Protein Complex
Plant Development
proteins
Sunlight
photosystem I
Solar System
protein degradation
Growth and Development
Arabidopsis

Keywords

  • Arabidopsis
  • Chloroplast
  • Fluctuating lights
  • Photosynthesis
  • PSB33
  • PSII
  • Quality control of PSII
  • State transition

Cite this

Fristedt, R., Trotta, A., Suorsa, M., Nilsson, A. K., Croce, R., Aro, E. M., & Lundin, B. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany, 68(15), 4281-4293. https://doi.org/10.1093/jxb/erx218
Fristedt, Rikard ; Trotta, Andrea ; Suorsa, Marjaana ; Nilsson, Anders K. ; Croce, Roberta ; Aro, Eva Mari ; Lundin, Björn. / PSB33 sustains photosystem II D1 protein under fluctuating light conditions. In: Journal of Experimental Botany. 2017 ; Vol. 68, No. 15. pp. 4281-4293.
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abstract = "On Earth, solar irradiance varies as the sun rises and sets over the horizon, and sunlight is thus in constant fluctuation, following a slow dark–low–high–low–dark curve. Optimal plant growth and development are dependent on the capacity of plants to acclimate and regulate photosynthesis in response to these changes of light. Little is known of regulative processes for photosynthesis during nocturnal events. The nucleus-encoded plant lineage-specific protein PSB33 has been described as stabilizing the photosystem II complex, especially under light stress conditions, and plants lacking PSB33 have a dysfunctional state transition. To clarify the localization and function of this protein, we used phenomic, biochemical and proteomics approaches in the model plant Arabidopsis. We report that PSB33 is predominantly located in non-appressed thylakoid regions and dynamically associates with a thylakoid protein complex in a light-dependent manner. Moreover, plants lacking PSB33 show an accelerated D1 protein degradation in nocturnal periods, and show severely stunted growth when challenged with fluctuating light. We further show that the function of PSB33 precedes the STN7 kinase to regulate or balance the excitation energy of photosystems I and II in fluctuating light conditions.",
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Fristedt, R, Trotta, A, Suorsa, M, Nilsson, AK, Croce, R, Aro, EM & Lundin, B 2017, 'PSB33 sustains photosystem II D1 protein under fluctuating light conditions' Journal of Experimental Botany, vol. 68, no. 15, pp. 4281-4293. https://doi.org/10.1093/jxb/erx218

PSB33 sustains photosystem II D1 protein under fluctuating light conditions. / Fristedt, Rikard; Trotta, Andrea; Suorsa, Marjaana; Nilsson, Anders K.; Croce, Roberta; Aro, Eva Mari; Lundin, Björn.

In: Journal of Experimental Botany, Vol. 68, No. 15, 10.07.2017, p. 4281-4293.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Fristedt, Rikard

AU - Trotta, Andrea

AU - Suorsa, Marjaana

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AU - Aro, Eva Mari

AU - Lundin, Björn

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AB - On Earth, solar irradiance varies as the sun rises and sets over the horizon, and sunlight is thus in constant fluctuation, following a slow dark–low–high–low–dark curve. Optimal plant growth and development are dependent on the capacity of plants to acclimate and regulate photosynthesis in response to these changes of light. Little is known of regulative processes for photosynthesis during nocturnal events. The nucleus-encoded plant lineage-specific protein PSB33 has been described as stabilizing the photosystem II complex, especially under light stress conditions, and plants lacking PSB33 have a dysfunctional state transition. To clarify the localization and function of this protein, we used phenomic, biochemical and proteomics approaches in the model plant Arabidopsis. We report that PSB33 is predominantly located in non-appressed thylakoid regions and dynamically associates with a thylakoid protein complex in a light-dependent manner. Moreover, plants lacking PSB33 show an accelerated D1 protein degradation in nocturnal periods, and show severely stunted growth when challenged with fluctuating light. We further show that the function of PSB33 precedes the STN7 kinase to regulate or balance the excitation energy of photosystems I and II in fluctuating light conditions.

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