Purification of the autotransporter protein Hbp of Escherichia coli.

S.J.M. van Dooren; J.R.H. Tame; S. Luirink; B. Oudega; B.R. Otto

doi:10.1016/S0378-1097(01)00454-2

Purification of the autotransporter protein Hbp of Escherichia coli.

S.J.M. van Dooren, J.R.H. Tame, S. Luirink, B. Oudega, B.R. Otto

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The enzyme Hbp (hemoglobin protease) of the pathogenic Escherichia coli strain EB1 has been purified to homogeneity by gel filtration chromatography. The purified protein is capable of binding heme and shows hemoglobin protease activity. Our method of purification is applicable not only to Hbp but also to other autotransporter proteins and will contribute to a better understanding of the function-structure relationship of this family of proteins. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	147-150
Number of pages	3
Journal	FEMS Microbiology Letters
Volume	205
DOIs	https://doi.org/10.1016/S0378-1097(01)00454-2
Publication status	Published - 2001

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10.1016/S0378-1097(01)00454-2

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title = "Purification of the autotransporter protein Hbp of Escherichia coli.",

abstract = "The enzyme Hbp (hemoglobin protease) of the pathogenic Escherichia coli strain EB1 has been purified to homogeneity by gel filtration chromatography. The purified protein is capable of binding heme and shows hemoglobin protease activity. Our method of purification is applicable not only to Hbp but also to other autotransporter proteins and will contribute to a better understanding of the function-structure relationship of this family of proteins. {\textcopyright} 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",

author = "{van Dooren}, S.J.M. and J.R.H. Tame and S. Luirink and B. Oudega and B.R. Otto",

year = "2001",

doi = "10.1016/S0378-1097(01)00454-2",

language = "English",

volume = "205",

pages = "147--150",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Purification of the autotransporter protein Hbp of Escherichia coli.

AU - van Dooren, S.J.M.

AU - Tame, J.R.H.

AU - Luirink, S.

AU - Oudega, B.

AU - Otto, B.R.

PY - 2001

Y1 - 2001

N2 - The enzyme Hbp (hemoglobin protease) of the pathogenic Escherichia coli strain EB1 has been purified to homogeneity by gel filtration chromatography. The purified protein is capable of binding heme and shows hemoglobin protease activity. Our method of purification is applicable not only to Hbp but also to other autotransporter proteins and will contribute to a better understanding of the function-structure relationship of this family of proteins. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - The enzyme Hbp (hemoglobin protease) of the pathogenic Escherichia coli strain EB1 has been purified to homogeneity by gel filtration chromatography. The purified protein is capable of binding heme and shows hemoglobin protease activity. Our method of purification is applicable not only to Hbp but also to other autotransporter proteins and will contribute to a better understanding of the function-structure relationship of this family of proteins. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

U2 - 10.1016/S0378-1097(01)00454-2

DO - 10.1016/S0378-1097(01)00454-2

M3 - Article

SN - 0378-1097

VL - 205

SP - 147

EP - 150

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

ER -

Purification of the autotransporter protein Hbp of Escherichia coli.

Abstract

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