Pushing the limits of what is achievable in protein-DNA docking: benchmarking HADDOCK's performance

Marc van Dijk, Alexandre M.J.J Bonvin

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The intrinsic flexibility of DNA and the difficulty of identifying its interaction surface have long been challenges that prevented the development of efficient protein-DNA docking methods. We have demonstrated the ability our flexible data-driven docking method HADDOCK to deal with these before, by using custom-built DNA structural models. Here we put our method to the test on a set of 47 complexes from the protein-DNA docking benchmark. We show that HADDOCK is able to predict many of the specific DNA conformational changes required to assemble the interface(s). Our DNA analysis and modelling procedure captures the bend and twist motions occurring upon complex formation and uses these to generate custom-built DNA structural models, more closely resembling the bound form, for use in a second docking round. We achieve throughout the benchmark an overall success rate of 94% of one-star solutions or higher (interface root mean square deviation ≤4 A and fraction of native contacts >10%) according to CAPRI criteria. Our improved protocol successfully predicts even the challenging protein-DNA complexes in the benchmark. Finally, our method is the first to readily dock multiple molecules (N > 2) simultaneously, pushing the limits of what is currently achievable in the field of protein-DNA docking.

Original languageEnglish
Pages (from-to)5634-47
Number of pages14
JournalNucleic Acids Research
Volume38
Issue number17
DOIs
Publication statusPublished - Sep 2010

Keywords

  • Benchmarking
  • DNA
  • DNA-Binding Proteins
  • Models, Molecular
  • Nucleic Acid Conformation
  • Protein Conformation
  • Software
  • Evaluation Studies
  • Journal Article
  • Research Support, Non-U.S. Gov't

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