TY - JOUR
T1 - Quenched phosphorescence as alternative detection mode in the chiral separation of methotrexate by electrokinetic chromatography.
AU - Castro, M.G.
AU - Lammers, I.
AU - Buijs, J.B.
AU - Gooijer, C.
AU - Ariese, F.
PY - 2011
Y1 - 2011
N2 - Quenched phosphorescence was used, for the first time, as detection mode in the chiral separation of methotrexate (MTX) enantiomers by electrokinetic chromatography. The detection is based on dynamic quenching of the strong emission of the phosphorophore 1-bromo-4-naphthalene sulfonic acid (BrNS) by MTX under deoxygenated conditions. The use of a background electrolyte with 3 mg/mL 2-hydroxypropyl-β-cyclodextrin and 20% MeOH in 25 mM phosphate buffer (pH 7.0) and an applied voltage of 30 kV allowed the separation of l-MTX and its enantiomeric impurity d-MTX with sufficient resolution. In the presence of 1 mM BrNS, a detection limit of 3.2 × 10
AB - Quenched phosphorescence was used, for the first time, as detection mode in the chiral separation of methotrexate (MTX) enantiomers by electrokinetic chromatography. The detection is based on dynamic quenching of the strong emission of the phosphorophore 1-bromo-4-naphthalene sulfonic acid (BrNS) by MTX under deoxygenated conditions. The use of a background electrolyte with 3 mg/mL 2-hydroxypropyl-β-cyclodextrin and 20% MeOH in 25 mM phosphate buffer (pH 7.0) and an applied voltage of 30 kV allowed the separation of l-MTX and its enantiomeric impurity d-MTX with sufficient resolution. In the presence of 1 mM BrNS, a detection limit of 3.2 × 10
UR - https://www.scopus.com/pages/publications/85027928891
UR - https://www.scopus.com/inward/citedby.url?scp=85027928891&partnerID=8YFLogxK
U2 - 10.1007/s00216-011-4991-6
DO - 10.1007/s00216-011-4991-6
M3 - Article
SN - 1618-2642
VL - 400
SP - 2913
EP - 2919
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 9
ER -
