RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana

Rikard Fristedt*, Chen Hu, Nicole Wheatley, Laura M. Roy, Rebekka M. Wachter, Linda Savage, Jeremy Harbinson, David M. Kramer, Sabeeha S. Merchant, Todd Yeates, Roberta Croce

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO2) and ribulose-1,5-bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to pterin-4α-carbinolamine dehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD-like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana.

Original languageEnglish
Pages (from-to)146-156
Number of pages11
JournalPlant Journal
Volume94
Issue number1
Early online date3 Feb 2018
DOIs
Publication statusPublished - Apr 2018

Keywords

  • abscisic acid
  • Atg5 g51110
  • chloroplast
  • RAF2
  • RuBisCO
  • RuBisCO aggregation
  • RuBisCO assembly factor
  • SDIRIP1

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