Raman spectroscopy for future planetary exploration: photodegradation, self-absorption and quantification of carotenoids in microorganisms and mineral matrices

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Carotenoids are among the key biomarkers in the search for life on other planets, and non-destructive Raman spectroscopy on future rover missions is a potential sensitive detection method, especially under resonant conditions. In this research, reflectance spectra of minerals and microorganisms were measured using ultraviolet/visible diffuse reflectance spectroscopy in order to evaluate potential resonance Raman conditions and the possible degree of sample damage during laser irradiation. We report a photodegradation and semi-quantitative Raman study of β-carotene and the carotenoid-containing extremophile Deinococcus radiodurans mixed with calcite at excitation wavelengths of 440nm, 532nm and 785nm. A different type of carotenoid was detected in a culture of Chroococcidiopsis. Carotenoids embedded in bacterial membranes were found to be less sensitive to photodegradation than in a mineral matrix. Corrections for self-absorption effects were performed using the 1085cm
Original languageEnglish
Pages (from-to)856-862
JournalJournal of Raman Spectroscopy
Volume46
Issue number10
DOIs
Publication statusPublished - 2015

Fingerprint

Photodegradation
Carotenoids
Microorganisms
Minerals
Raman spectroscopy
Calcium Carbonate
Biomarkers
Planets
Laser beam effects
Calcite
Spectroscopy
Membranes
Wavelength

Cite this

@article{c9b0e377a44f4f668ebce8058b2c4c0e,
title = "Raman spectroscopy for future planetary exploration: photodegradation, self-absorption and quantification of carotenoids in microorganisms and mineral matrices",
abstract = "Carotenoids are among the key biomarkers in the search for life on other planets, and non-destructive Raman spectroscopy on future rover missions is a potential sensitive detection method, especially under resonant conditions. In this research, reflectance spectra of minerals and microorganisms were measured using ultraviolet/visible diffuse reflectance spectroscopy in order to evaluate potential resonance Raman conditions and the possible degree of sample damage during laser irradiation. We report a photodegradation and semi-quantitative Raman study of β-carotene and the carotenoid-containing extremophile Deinococcus radiodurans mixed with calcite at excitation wavelengths of 440nm, 532nm and 785nm. A different type of carotenoid was detected in a culture of Chroococcidiopsis. Carotenoids embedded in bacterial membranes were found to be less sensitive to photodegradation than in a mineral matrix. Corrections for self-absorption effects were performed using the 1085cm",
author = "J.H. Hooijschuur and M.F.C. Verkaaik and G.R. Davies and F. Ariese",
year = "2015",
doi = "10.1002/jrs.4647",
language = "English",
volume = "46",
pages = "856--862",
journal = "Journal of Raman Spectroscopy",
issn = "0377-0486",
publisher = "John Wiley and Sons Ltd",
number = "10",

}

TY - JOUR

T1 - Raman spectroscopy for future planetary exploration: photodegradation, self-absorption and quantification of carotenoids in microorganisms and mineral matrices

AU - Hooijschuur, J.H.

AU - Verkaaik, M.F.C.

AU - Davies, G.R.

AU - Ariese, F.

PY - 2015

Y1 - 2015

N2 - Carotenoids are among the key biomarkers in the search for life on other planets, and non-destructive Raman spectroscopy on future rover missions is a potential sensitive detection method, especially under resonant conditions. In this research, reflectance spectra of minerals and microorganisms were measured using ultraviolet/visible diffuse reflectance spectroscopy in order to evaluate potential resonance Raman conditions and the possible degree of sample damage during laser irradiation. We report a photodegradation and semi-quantitative Raman study of β-carotene and the carotenoid-containing extremophile Deinococcus radiodurans mixed with calcite at excitation wavelengths of 440nm, 532nm and 785nm. A different type of carotenoid was detected in a culture of Chroococcidiopsis. Carotenoids embedded in bacterial membranes were found to be less sensitive to photodegradation than in a mineral matrix. Corrections for self-absorption effects were performed using the 1085cm

AB - Carotenoids are among the key biomarkers in the search for life on other planets, and non-destructive Raman spectroscopy on future rover missions is a potential sensitive detection method, especially under resonant conditions. In this research, reflectance spectra of minerals and microorganisms were measured using ultraviolet/visible diffuse reflectance spectroscopy in order to evaluate potential resonance Raman conditions and the possible degree of sample damage during laser irradiation. We report a photodegradation and semi-quantitative Raman study of β-carotene and the carotenoid-containing extremophile Deinococcus radiodurans mixed with calcite at excitation wavelengths of 440nm, 532nm and 785nm. A different type of carotenoid was detected in a culture of Chroococcidiopsis. Carotenoids embedded in bacterial membranes were found to be less sensitive to photodegradation than in a mineral matrix. Corrections for self-absorption effects were performed using the 1085cm

U2 - 10.1002/jrs.4647

DO - 10.1002/jrs.4647

M3 - Article

VL - 46

SP - 856

EP - 862

JO - Journal of Raman Spectroscopy

JF - Journal of Raman Spectroscopy

SN - 0377-0486

IS - 10

ER -