Rat oxytocin receptor in brain, pituitary, mammary gland, and uterus: partial sequence and immunocytochemical localization

R A Adan, F W Van Leeuwen, M A Sonnemans, M Brouns, G Hoffman, J G Verbalis, J P Burbach

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Partial complementary DNAs of an oxytocin (OT) receptor were cloned from rat brain and uterus. The complementary DNAs encoded for the same amino acid sequence, which showed a high degree of homology with the human and porcine uterine OT receptors, except for a region in the third intracellular loop. Antibodies were raised against nonoverlapping sequences of the third intracellular loop of this rat OT receptor. Using these antisera, OT receptor expression was demonstrated in the brain, pituitary, mammary gland, and uterus by immunocytochemistry. In the brain, several areas including the ventromedial hypothalamus, the bed nucleus of the stria terminalis, the ventral pallidum, the paraventricular nucleus, and the dorsal part of the supraoptic nucleus, demonstrated OT-receptor immunoreactivity. However, no immunoreactivity was detected in two areas of the brain known to contain dense OT-binding sites by receptor autoradiography studies: the ventral hippocampus and the central nucleus of the amygdala. In the pituitary, both the anterior and posterior lobes were positive for OT receptor immunoreactivity, whereas the intermediate lobe was negative. These results demonstrate that the same receptor type is expressed in both peripheral OT target tissues and the brain, and also suggest the possibility that a different OT receptor subtype may be present in some areas of the brain.

    Original languageEnglish
    Pages (from-to)4022-4028
    Number of pages7
    JournalEndocrinology
    Volume136
    Issue number9
    DOIs
    Publication statusPublished - Sep 1995

    Keywords

    • Amino Acid Sequence
    • Amygdalin/chemistry
    • Animals
    • Base Sequence
    • Brain Chemistry
    • DNA, Complementary/analysis
    • Female
    • Hippocampus/chemistry
    • Immune Sera/analysis
    • Immunohistochemistry
    • Mammary Glands, Animal/chemistry
    • Molecular Sequence Data
    • Oligonucleotides/chemistry
    • Paraventricular Hypothalamic Nucleus/chemistry
    • Pituitary Gland/chemistry
    • Polymerase Chain Reaction
    • Rats
    • Receptors, Oxytocin/analysis
    • Sequence Homology, Amino Acid
    • Supraoptic Nucleus/chemistry
    • Uterus/chemistry
    • Ventromedial Hypothalamic Nucleus/chemistry

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  • Cite this

    Adan, R. A., Van Leeuwen, F. W., Sonnemans, M. A., Brouns, M., Hoffman, G., Verbalis, J. G., & Burbach, J. P. (1995). Rat oxytocin receptor in brain, pituitary, mammary gland, and uterus: partial sequence and immunocytochemical localization. Endocrinology, 136(9), 4022-4028. https://doi.org/10.1210/endo.136.9.7649111