Rat oxytocin receptor in brain, pituitary, mammary gland, and uterus: partial sequence and immunocytochemical localization

R A Adan, F W Van Leeuwen, M A Sonnemans, M Brouns, G Hoffman, J G Verbalis, J P Burbach

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Partial complementary DNAs of an oxytocin (OT) receptor were cloned from rat brain and uterus. The complementary DNAs encoded for the same amino acid sequence, which showed a high degree of homology with the human and porcine uterine OT receptors, except for a region in the third intracellular loop. Antibodies were raised against nonoverlapping sequences of the third intracellular loop of this rat OT receptor. Using these antisera, OT receptor expression was demonstrated in the brain, pituitary, mammary gland, and uterus by immunocytochemistry. In the brain, several areas including the ventromedial hypothalamus, the bed nucleus of the stria terminalis, the ventral pallidum, the paraventricular nucleus, and the dorsal part of the supraoptic nucleus, demonstrated OT-receptor immunoreactivity. However, no immunoreactivity was detected in two areas of the brain known to contain dense OT-binding sites by receptor autoradiography studies: the ventral hippocampus and the central nucleus of the amygdala. In the pituitary, both the anterior and posterior lobes were positive for OT receptor immunoreactivity, whereas the intermediate lobe was negative. These results demonstrate that the same receptor type is expressed in both peripheral OT target tissues and the brain, and also suggest the possibility that a different OT receptor subtype may be present in some areas of the brain.

Original languageEnglish
Pages (from-to)4022-8
Number of pages7
JournalEndocrinology
Volume136
Issue number9
DOIs
Publication statusPublished - Sep 1995

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Oxytocin Receptors
Pituitary Gland
Human Mammary Glands
Uterus
Brain
Oxytocin
Complementary DNA
Supraoptic Nucleus
Septal Nuclei
Paraventricular Hypothalamic Nucleus
Autoradiography
Hypothalamus
rat oxytocin receptors
Immune Sera
Amino Acid Sequence
Hippocampus
Swine
Immunohistochemistry
Binding Sites
Antibodies

Keywords

  • Amino Acid Sequence
  • Amygdalin/chemistry
  • Animals
  • Base Sequence
  • Brain Chemistry
  • DNA, Complementary/analysis
  • Female
  • Hippocampus/chemistry
  • Immune Sera/analysis
  • Immunohistochemistry
  • Mammary Glands, Animal/chemistry
  • Molecular Sequence Data
  • Oligonucleotides/chemistry
  • Paraventricular Hypothalamic Nucleus/chemistry
  • Pituitary Gland/chemistry
  • Polymerase Chain Reaction
  • Rats
  • Receptors, Oxytocin/analysis
  • Sequence Homology, Amino Acid
  • Supraoptic Nucleus/chemistry
  • Uterus/chemistry
  • Ventromedial Hypothalamic Nucleus/chemistry

Cite this

Adan, R. A., Van Leeuwen, F. W., Sonnemans, M. A., Brouns, M., Hoffman, G., Verbalis, J. G., & Burbach, J. P. (1995). Rat oxytocin receptor in brain, pituitary, mammary gland, and uterus: partial sequence and immunocytochemical localization. Endocrinology, 136(9), 4022-8. https://doi.org/10.1210/endo.136.9.7649111
Adan, R A ; Van Leeuwen, F W ; Sonnemans, M A ; Brouns, M ; Hoffman, G ; Verbalis, J G ; Burbach, J P. / Rat oxytocin receptor in brain, pituitary, mammary gland, and uterus : partial sequence and immunocytochemical localization. In: Endocrinology. 1995 ; Vol. 136, No. 9. pp. 4022-8.
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abstract = "Partial complementary DNAs of an oxytocin (OT) receptor were cloned from rat brain and uterus. The complementary DNAs encoded for the same amino acid sequence, which showed a high degree of homology with the human and porcine uterine OT receptors, except for a region in the third intracellular loop. Antibodies were raised against nonoverlapping sequences of the third intracellular loop of this rat OT receptor. Using these antisera, OT receptor expression was demonstrated in the brain, pituitary, mammary gland, and uterus by immunocytochemistry. In the brain, several areas including the ventromedial hypothalamus, the bed nucleus of the stria terminalis, the ventral pallidum, the paraventricular nucleus, and the dorsal part of the supraoptic nucleus, demonstrated OT-receptor immunoreactivity. However, no immunoreactivity was detected in two areas of the brain known to contain dense OT-binding sites by receptor autoradiography studies: the ventral hippocampus and the central nucleus of the amygdala. In the pituitary, both the anterior and posterior lobes were positive for OT receptor immunoreactivity, whereas the intermediate lobe was negative. These results demonstrate that the same receptor type is expressed in both peripheral OT target tissues and the brain, and also suggest the possibility that a different OT receptor subtype may be present in some areas of the brain.",
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Rat oxytocin receptor in brain, pituitary, mammary gland, and uterus : partial sequence and immunocytochemical localization. / Adan, R A; Van Leeuwen, F W; Sonnemans, M A; Brouns, M; Hoffman, G; Verbalis, J G; Burbach, J P.

In: Endocrinology, Vol. 136, No. 9, 09.1995, p. 4022-8.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Rat oxytocin receptor in brain, pituitary, mammary gland, and uterus

T2 - partial sequence and immunocytochemical localization

AU - Adan, R A

AU - Van Leeuwen, F W

AU - Sonnemans, M A

AU - Brouns, M

AU - Hoffman, G

AU - Verbalis, J G

AU - Burbach, J P

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AB - Partial complementary DNAs of an oxytocin (OT) receptor were cloned from rat brain and uterus. The complementary DNAs encoded for the same amino acid sequence, which showed a high degree of homology with the human and porcine uterine OT receptors, except for a region in the third intracellular loop. Antibodies were raised against nonoverlapping sequences of the third intracellular loop of this rat OT receptor. Using these antisera, OT receptor expression was demonstrated in the brain, pituitary, mammary gland, and uterus by immunocytochemistry. In the brain, several areas including the ventromedial hypothalamus, the bed nucleus of the stria terminalis, the ventral pallidum, the paraventricular nucleus, and the dorsal part of the supraoptic nucleus, demonstrated OT-receptor immunoreactivity. However, no immunoreactivity was detected in two areas of the brain known to contain dense OT-binding sites by receptor autoradiography studies: the ventral hippocampus and the central nucleus of the amygdala. In the pituitary, both the anterior and posterior lobes were positive for OT receptor immunoreactivity, whereas the intermediate lobe was negative. These results demonstrate that the same receptor type is expressed in both peripheral OT target tissues and the brain, and also suggest the possibility that a different OT receptor subtype may be present in some areas of the brain.

KW - Amino Acid Sequence

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KW - Animals

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KW - Brain Chemistry

KW - DNA, Complementary/analysis

KW - Female

KW - Hippocampus/chemistry

KW - Immune Sera/analysis

KW - Immunohistochemistry

KW - Mammary Glands, Animal/chemistry

KW - Molecular Sequence Data

KW - Oligonucleotides/chemistry

KW - Paraventricular Hypothalamic Nucleus/chemistry

KW - Pituitary Gland/chemistry

KW - Polymerase Chain Reaction

KW - Rats

KW - Receptors, Oxytocin/analysis

KW - Sequence Homology, Amino Acid

KW - Supraoptic Nucleus/chemistry

KW - Uterus/chemistry

KW - Ventromedial Hypothalamic Nucleus/chemistry

U2 - 10.1210/endo.136.9.7649111

DO - 10.1210/endo.136.9.7649111

M3 - Article

VL - 136

SP - 4022

EP - 4028

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

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ER -