Real-time measurements of the redox states of c-type cytochromes in electroactive biofilms: a confocal resonance Raman microscopy study

B. Virdis, D. Millo, B.C. Donose, D.J. Batstone

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Confocal Resonance Raman Microscopy (CRRM) was used to probe variations of redox state of c-type cytochromes embedded in living mixed-culture electroactive biofilms exposed to different electrode polarizations, under potentiostatic and potentiodynamic conditions. In the absence of the metabolic substrate acetate, the redox state of cytochromes followed the application of reducing and oxidizing electrode potentials. Real-time monitoring of the redox state of cytochromes during cyclic voltammetry (CV) in a potential window where cytochromes reduction occurs, evidenced a measurable time delay between the oxidation of redox cofactors probed by CV at the electrode interface, and oxidation of distal cytochromes probed by CRRM. This delay was used to tentatively estimate the diffusivity of electrons through the biofilm. In the presence of acetate, the resonance Raman spectra of young (10 days, j = 208±49 μA cm
Original languageEnglish
Pages (from-to)e89918
JournalPLoS ONE
Volume2014
Issue number9
DOIs
Publication statusPublished - 2014

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