Recent advances in liquid-phase separations for clinical metabolomics

Isabelle Kohler*, Martin Giera

*Corresponding author for this work

Research output: Contribution to JournalReview articleAcademicpeer-review


Over the last decades, several technological improvements have been achieved in liquid-based separation techniques, notably, with the advent of fully porous sub-2 μm particles and superficially porous sub-3 μm particles, the comeback of supercritical fluid chromatography, and the development of alternative chromatographic modes such as hydrophilic interaction chromatography. Combined with mass spectrometry, these techniques have demonstrated their added value, substantially increasing separation efficiency, selectivity, and speed of analysis. These benefits are essential in modern clinical metabolomics typically involving the study of large-scale sample cohorts and the analysis of thousands of metabolites showing extensive differences in physicochemical properties. This review presents a brief overview of the recent developments in liquid-phase separation sciences in the context of clinical metabolomics, focusing on increased throughput as well as metabolite coverage. Relevant metabolomics applications highlighting the benefits of ultra-high performance liquid chromatography, core–shell technology, high-temperature liquid chromatography, capillary electrophoresis, supercritical fluid chromatography, and hydrophilic interaction chromatography are discussed.

Original languageEnglish
Pages (from-to)93-108
Number of pages16
JournalJournal of Separation Science
Issue number1
Publication statusPublished - 1 Jan 2017
Externally publishedYes


  • Capillary electrophoresis
  • Clinical metabolomics
  • High-throughput analysis
  • Liquid chromatography
  • Supercritical fluid chromatography


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