Recent developments in protein-ligand affinity mass spectrometry

N. Jonker, J. Kool, H. Irth, W.M.A. Niessen

Research output: Contribution to JournalArticleAcademicpeer-review

160 Downloads (Pure)


This review provides an overview of direct and indirect technologies to screen protein-ligand interactions with mass spectrometry. These technologies have as a key feature the selection or affinity purification of ligands in mixtures prior to detection. Specific fields of interest for these technologies are metabolic profiling of bioactive metabolites, natural extract screening, and the screening of libraries for bioactives, such as parallel synthesis libraries and small combichem libraries. The review addresses the principles of each of the methods discussed, with a focus on developments in recent years, and the applicability of the methods to lead generation and development in drug discovery. © The Author(s) 2010.
Original languageEnglish
Pages (from-to)2669-2681
JournalAnalytical and Bioanalytical Chemistry
Issue number8
Publication statusPublished - 2011


Dive into the research topics of 'Recent developments in protein-ligand affinity mass spectrometry'. Together they form a unique fingerprint.

Cite this