Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping

Nelda Antonovaite; Steven V. Beekmans; Elly M. Hol; Wytse J. Wadman; Davide Iannuzzi

doi:10.1038/s41598-018-31035-y

Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping

Nelda Antonovaite^*, Steven V. Beekmans, Elly M. Hol, Wytse J. Wadman, Davide Iannuzzi

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The mechanical properties of brain tissue play a pivotal role in neurodevelopment and neurological disorders. Yet, at present, there is no consensus on how the different structural parts of the tissue contribute to its stiffness variations. Here, we have gathered depth-controlled indentation viscoelasticity maps of the hippocampus of acute horizontal live mouse brain slices. Our results confirm the highly viscoelestic nature of brain tissue. We further show that the mechanical properties are non-uniform and at least related to differences in morphological composition. Interestingly, areas with higher nuclear density appear to be softer than areas with lower nuclear density.

Original language	English
Article number	12517
Pages (from-to)	1-11
Number of pages	11
Journal	Scientific Reports
Volume	8
DOIs	https://doi.org/10.1038/s41598-018-31035-y
Publication status	Published - 21 Aug 2018

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title = "Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping",

abstract = "The mechanical properties of brain tissue play a pivotal role in neurodevelopment and neurological disorders. Yet, at present, there is no consensus on how the different structural parts of the tissue contribute to its stiffness variations. Here, we have gathered depth-controlled indentation viscoelasticity maps of the hippocampus of acute horizontal live mouse brain slices. Our results confirm the highly viscoelestic nature of brain tissue. We further show that the mechanical properties are non-uniform and at least related to differences in morphological composition. Interestingly, areas with higher nuclear density appear to be softer than areas with lower nuclear density.",

author = "Nelda Antonovaite and Beekmans, {Steven V.} and Hol, {Elly M.} and Wadman, {Wytse J.} and Davide Iannuzzi",

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doi = "10.1038/s41598-018-31035-y",

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AU - Beekmans, Steven V.

AU - Hol, Elly M.

AU - Wadman, Wytse J.

AU - Iannuzzi, Davide

PY - 2018/8/21

Y1 - 2018/8/21

N2 - The mechanical properties of brain tissue play a pivotal role in neurodevelopment and neurological disorders. Yet, at present, there is no consensus on how the different structural parts of the tissue contribute to its stiffness variations. Here, we have gathered depth-controlled indentation viscoelasticity maps of the hippocampus of acute horizontal live mouse brain slices. Our results confirm the highly viscoelestic nature of brain tissue. We further show that the mechanical properties are non-uniform and at least related to differences in morphological composition. Interestingly, areas with higher nuclear density appear to be softer than areas with lower nuclear density.

AB - The mechanical properties of brain tissue play a pivotal role in neurodevelopment and neurological disorders. Yet, at present, there is no consensus on how the different structural parts of the tissue contribute to its stiffness variations. Here, we have gathered depth-controlled indentation viscoelasticity maps of the hippocampus of acute horizontal live mouse brain slices. Our results confirm the highly viscoelestic nature of brain tissue. We further show that the mechanical properties are non-uniform and at least related to differences in morphological composition. Interestingly, areas with higher nuclear density appear to be softer than areas with lower nuclear density.

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Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping

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