Regulation of nitrogen fixation from free-living organisms in soil and leaf litter of two tropical forests of the Guiana shield

Leandro Van Langenhove*, Thomas Depaepe, Sara Vicca, Joke van den Berge, Clement Stahl, Elodie Courtois, James Weedon, Ifigenia Urbina, Oriol Grau, Dolores Asensio, Josep Peñuelas, Pascal Boeckx, Andreas Richter, Dominique Van Der Straeten, Ivan A. Janssens

*Corresponding author for this work

    Research output: Contribution to JournalArticleAcademicpeer-review


    Background and aims: Biological fixation of atmospheric nitrogen (N 2 ) is the main pathway for introducing N into unmanaged ecosystems. While recent estimates suggest that free-living N fixation (FLNF) accounts for the majority of N fixed in mature tropical forests, the controls governing this process are not completely understood. The aim of this study was to quantify FLNF rates and determine its drivers in two tropical pristine forests of French Guiana. Methods: We used the acetylene reduction assay to measure FLNF rates at two sites, in two seasons and along three topographical positions, and used regression analyses to identify which edaphic explanatory variables, including carbon (C), nitrogen (N), phosphorus (P) and molybdenum (Mo) content, pH, water and available N and P, explained most of the variation in FLNF rates. Results: Overall, FLNF rates were lower than measured in tropical systems elsewhere. In soils seasonal variability was small and FLNF rates differed among topographies at only one site. Water, P and pH explained 24% of the variation. In leaf litter, FLNF rates differed seasonally, without site or topographical differences. Water, C, N and P explained 46% of the observed variation. We found no regulatory role of Mo at our sites. Conclusions: Rates of FLNF were low in primary rainforest on poor soils on the Guiana shield. Water was the most important rate-regulating factor and FLNF increased with increasing P, but decreased with increasing N. Our results support the general assumption that N fixation in tropical lowland forests is limited by P availability.

    Original languageEnglish
    Pages (from-to)1–18
    JournalPlant and Soil
    Issue number1-2
    Early online date1 Apr 2019
    Publication statusPublished - 1 Apr 2019


    This research was supported by the European Research Council Synergy grant ERC-2013-SyG. 610028-IMBALANCE-P. We thank the staff of the Nouragues station, managed by USR mixte LEEISA. (CNRS; Cayenne), and the Paracou station, managed by UMR Ecofog (CIRAD, INRA; Kourou). Both research stations received support from “Investissement d’Avenir” grants managed by Agence Nationale de la Recherche (CEBA: ANR-10-LABX-25-01,ANAEE France: ANR-11-INBS-0001). We thank Tom Van der Spiet, Anne Cools and Miguel Portillo Estrada for their help with analyses and Philippe Gaucher, Nadine Calluy, Lore Verryckt and Kevin Van Sundert for their help with logistics and statistics.

    FundersFunder number
    UMR Ecofog
    Seventh Framework Programme610028
    European Research CouncilERC-2013-SyG
    Agence Nationale de la RechercheANR-11-INBS-0001, ANR-10-LABX-25-01
    Centre National de la Recherche Scientifique
    Institut National de la Recherche Agronomique
    Centre de coopération internationale en recherche agronomique pour le développement


      • Free-living nitrogen fixation
      • French Guiana
      • Molybdenum
      • Nutrients
      • Phosphorus
      • Tropical forest


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