Reinitiated viral RNA-dependent RNA polymerase resumes replication at a reduced rate

I. D. Vilfan, A. Candelli, S. Hage, A. P. Aalto, M. M. Poranen, D. H. Bamford, N.P. Dekker

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

RNA-dependent RNA polymerases (RdRP) form an important class of enzymes that is responsible for genome replication and transcription in RNA viruses and involved in the regulation of RNA interference in plants and fungi. The RdRP kinetics have been extensively studied, but pausing, an important regulatory mechanism for RNA polymerases that has also been implicated in RNA recombination, has not been considered. Here, we report that RdRP experience a dramatic, long-lived decrease in its elongation rate when it is reinitiated following stalling. The rate decrease has an intriguingly weak temperature dependence, is independent of both the nucleotide concentration during stalling and the length of the RNA transcribed prior to stalling; however it is sensitive to RNA structure. This allows us to delineate the potential factors underlying this irreversible conversion of the elongation complex to a less active mode. © 2008 The Author(s).
Original languageEnglish
Pages (from-to)7059-7067
JournalNucleic Acids Research
Volume36
Issue number22
DOIs
Publication statusPublished - 2008

Bibliographical note

Reinitiated viral RNA-dependent RNA polymerase resumes replication at a reduced rate

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