Relationships between microbial community structure and hydrochemistry in a landfill leachate-polluted aquifer.

W.F.M. Roling, B.M. van Breukelen, M. Braster, B Lin, H.W. van Verseveld

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Knowledge about the relationship between microbial community structure and hydrogeochemistry (e.g., pollution, redox and degradation processes) in landfill leachate-polluted aquifers is required to develop tools for predicting and monitoring natural attenuation. In this study analyses of pollutant and redox chemistry were conducted in parallel with culture-independent profiling of microbial communities present in a well-defined aquifer (Banisveld, The Netherlands). Degradation of organic contaminants occurred under iron-reducing conditions in the plume of pollution, while upstream of the landfill and above the plume denitrification was the dominant redox process. Beneath the plume iron reduction occurred. Numerical comparison of 16S ribosomal DNA (rDNA)-based denaturing gradient gel electrophoresis (DGGE) profiles of Bacteria and Archaea in 29 groundwater samples revealed a clear difference between the microbial community structures inside and outside the contaminant plume. A similar relationship was not evident in sediment samples. DGGE data were supported by sequencing cloned 16S rDNA. Upstream of the landfill members of the β subclass of the class Proteobacteria (β-proteobacteria) dominated. This group was not encountered beneath the landfill, where gram-positive bacteria dominated. Further downstream the contribution of gram-positive bacteria to the clone library decreased, while the contribution of δ-proteobacteria strongly increased and β-proteobacteria reappeared. The β-proteobacteria (Acidovorax, Rhodoferax) differed considerably from those found upstream (Gallionella, Azoarcus). Direct comparisons of cloned 16S rDNA with bands in DGGE profiles revealed that the data from each analysis were comparable. A relationship was observed between the dominant redox processes and the bacteria identified. In the iron-reducing plume members of the family Geobacteraceae made a strong contribution to the microbial communities. Because the only known aromatic hydrocarbon-degrading, iron-reducing bacteria are Geobacter spp., their occurrence in landfill leachate-contaminated aquifers deserves more detailed consideration.
Original languageEnglish
Pages (from-to)4619-4629
JournalApplied and Environmental Microbiology
Volume67
Issue number10
DOIs
Publication statusPublished - 2001

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Chemical Water Pollutants
landfill leachates
Proteobacteria
hydrochemistry
Groundwater
Waste Disposal Facilities
Denaturing Gradient Gel Electrophoresis
aquifers
Oxidation-Reduction
microbial communities
microbial community
landfills
community structure
denaturing gradient gel electrophoresis
Iron
plume
Ribosomal DNA
aquifer
ribosomal DNA
iron

Cite this

Roling, W.F.M. ; van Breukelen, B.M. ; Braster, M. ; Lin, B ; van Verseveld, H.W. / Relationships between microbial community structure and hydrochemistry in a landfill leachate-polluted aquifer. In: Applied and Environmental Microbiology. 2001 ; Vol. 67, No. 10. pp. 4619-4629.
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abstract = "Knowledge about the relationship between microbial community structure and hydrogeochemistry (e.g., pollution, redox and degradation processes) in landfill leachate-polluted aquifers is required to develop tools for predicting and monitoring natural attenuation. In this study analyses of pollutant and redox chemistry were conducted in parallel with culture-independent profiling of microbial communities present in a well-defined aquifer (Banisveld, The Netherlands). Degradation of organic contaminants occurred under iron-reducing conditions in the plume of pollution, while upstream of the landfill and above the plume denitrification was the dominant redox process. Beneath the plume iron reduction occurred. Numerical comparison of 16S ribosomal DNA (rDNA)-based denaturing gradient gel electrophoresis (DGGE) profiles of Bacteria and Archaea in 29 groundwater samples revealed a clear difference between the microbial community structures inside and outside the contaminant plume. A similar relationship was not evident in sediment samples. DGGE data were supported by sequencing cloned 16S rDNA. Upstream of the landfill members of the β subclass of the class Proteobacteria (β-proteobacteria) dominated. This group was not encountered beneath the landfill, where gram-positive bacteria dominated. Further downstream the contribution of gram-positive bacteria to the clone library decreased, while the contribution of δ-proteobacteria strongly increased and β-proteobacteria reappeared. The β-proteobacteria (Acidovorax, Rhodoferax) differed considerably from those found upstream (Gallionella, Azoarcus). Direct comparisons of cloned 16S rDNA with bands in DGGE profiles revealed that the data from each analysis were comparable. A relationship was observed between the dominant redox processes and the bacteria identified. In the iron-reducing plume members of the family Geobacteraceae made a strong contribution to the microbial communities. Because the only known aromatic hydrocarbon-degrading, iron-reducing bacteria are Geobacter spp., their occurrence in landfill leachate-contaminated aquifers deserves more detailed consideration.",
author = "W.F.M. Roling and {van Breukelen}, B.M. and M. Braster and B Lin and {van Verseveld}, H.W.",
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Relationships between microbial community structure and hydrochemistry in a landfill leachate-polluted aquifer. / Roling, W.F.M.; van Breukelen, B.M.; Braster, M.; Lin, B; van Verseveld, H.W.

In: Applied and Environmental Microbiology, Vol. 67, No. 10, 2001, p. 4619-4629.

Research output: Contribution to JournalArticleAcademicpeer-review

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