Revealing traces of tumour DNA in the circulation: Towards clinical implementation

Jamie John Beagan

Revealing traces of tumour DNA in the circulation: Towards clinical implementation

Jamie John Beagan

Research output: PhD Thesis › PhD-Thesis - Research and graduation internal

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Abstract

This thesis bridges the gap between solid tissue and liquid biopsies and demonstrates the utility of circulating tumour (ct)DNA detection in an academic hospital. We show that routine mutation analysis of ctDNA is technically feasible and can inform clinical decisions in diverse subsets of cancer patients. However, the utility of ctDNA detection is currently hindered by poorly understood biological variables. Until these gaps in our knowledge are filled, ctDNA detection will be mostly restricted to a test of last resort or to support solid tissue biopsies.

Original language	English
Qualification	PhD
Awarding Institution	Vrije Universiteit Amsterdam
Supervisors/Advisors	Ylstra, B., Supervisor, - Pegtel, D.M., Co-supervisor, -
Award date	7 Apr 2022
Place of Publication	s.l.
Publisher	Ridderprint
Print ISBNs	9789464580938
Publication status	Published - 7 Apr 2022

Keywords

liquid biopsy
cell-free DNA
circulating tumour DNA
cancer
blood
lung cancer
colorectal cancer
droplet digital PCR
next generation sequencing
monitoring

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AB - This thesis bridges the gap between solid tissue and liquid biopsies and demonstrates the utility of circulating tumour (ct)DNA detection in an academic hospital. We show that routine mutation analysis of ctDNA is technically feasible and can inform clinical decisions in diverse subsets of cancer patients. However, the utility of ctDNA detection is currently hindered by poorly understood biological variables. Until these gaps in our knowledge are filled, ctDNA detection will be mostly restricted to a test of last resort or to support solid tissue biopsies.

KW - liquid biopsy

KW - cell-free DNA

KW - circulating tumour DNA

KW - cancer

KW - blood

KW - lung cancer

KW - colorectal cancer

KW - droplet digital PCR

KW - next generation sequencing

KW - monitoring

M3 - PhD-Thesis - Research and graduation internal

SN - 9789464580938

PB - Ridderprint

CY - s.l.

ER -

Revealing traces of tumour DNA in the circulation: Towards clinical implementation

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Keywords

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