Role of domains within the autotransporter Hbp/Tsh.

K. Nishimura, Y.H. Yoon, A. Kurihara, S. Unzai, S. Luirink, S.Y. Park, J.R.H. Tame

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The autotransporter Tsh (temperature-sensitive haem-agglutinin) secreted by avian pathogenic Escherichia coli was reported in 1994 and the almost identical Hbp (haemoglobin protease) was discovered some years later in isolates from patients suffering from peritoneal abscesses. However, the function of the protein remains uncertain. The crystal structure of Hbp shows that the protein carries a serine protease domain (domain 1) and a small domain of 75 residues called domain 2 which is inserted into the long β-helix characteristic of autotransporter passenger proteins. In this paper, domain 1 is shown to bind calcium, although metal ions binding to this site do not seem to regulate protease activity. Tsh has been reported to bind red cells and components of the extracellular matrix, but it is demonstrated that these properties are not a consequence of the presence of domain 2. © 2010 International Union of Crystallography.
Original languageEnglish
Pages (from-to)1295-1300
Number of pages6
JournalActa Crystallographica Section D. Biological Crystallography
Volume66
Issue number12
DOIs
Publication statusPublished - 2010

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Agglutinins
Heme
Hemoglobins
Peptide Hydrolases
Temperature
Crystallography
Proteins
Serine Proteases
Cellular Structures
Abscess
Extracellular Matrix
Metals
Binding Sites
Ions
Escherichia coli
Calcium
Type V Secretion Systems

Cite this

Nishimura, K., Yoon, Y. H., Kurihara, A., Unzai, S., Luirink, S., Park, S. Y., & Tame, J. R. H. (2010). Role of domains within the autotransporter Hbp/Tsh. Acta Crystallographica Section D. Biological Crystallography, 66(12), 1295-1300. https://doi.org/10.1107/S0907444910036966
Nishimura, K. ; Yoon, Y.H. ; Kurihara, A. ; Unzai, S. ; Luirink, S. ; Park, S.Y. ; Tame, J.R.H. / Role of domains within the autotransporter Hbp/Tsh. In: Acta Crystallographica Section D. Biological Crystallography. 2010 ; Vol. 66, No. 12. pp. 1295-1300.
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Nishimura, K, Yoon, YH, Kurihara, A, Unzai, S, Luirink, S, Park, SY & Tame, JRH 2010, 'Role of domains within the autotransporter Hbp/Tsh.' Acta Crystallographica Section D. Biological Crystallography, vol. 66, no. 12, pp. 1295-1300. https://doi.org/10.1107/S0907444910036966

Role of domains within the autotransporter Hbp/Tsh. / Nishimura, K.; Yoon, Y.H.; Kurihara, A.; Unzai, S.; Luirink, S.; Park, S.Y.; Tame, J.R.H.

In: Acta Crystallographica Section D. Biological Crystallography, Vol. 66, No. 12, 2010, p. 1295-1300.

Research output: Contribution to JournalArticleAcademicpeer-review

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