Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.

D.W. van Bloois; G.M. Koningstein; H. Bauerschmitt; J.M. Herrmann; S. Luirink

doi:10.1111/j.1742-4658.2007.06094.x

Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.

D.W. van Bloois, G.M. Koningstein, H. Bauerschmitt, J.M. Herrmann, S. Luirink

Molecular Microbiology

Research output: Contribution to Journal › Article › Academic

Abstract

Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. © 2007 The Authors.

Original language	English
Pages (from-to)	5704-5713
Journal	The FEBS Journal
Volume	274
Issue number	21
DOIs	https://doi.org/10.1111/j.1742-4658.2007.06094.x
Publication status	Published - 2007

Access to Document

10.1111/j.1742-4658.2007.06094.x

Fingerprint Dive into the research topics of 'Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.'. Together they form a unique fingerprint.

Cite this

@article{7d7d76e870c849b8b773b5d9c2b71e2a,

title = "Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.",

abstract = "Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. {\textcopyright} 2007 The Authors.",

author = "{van Bloois}, D.W. and G.M. Koningstein and H. Bauerschmitt and J.M. Herrmann and S. Luirink",

year = "2007",

doi = "10.1111/j.1742-4658.2007.06094.x",

language = "English",

volume = "274",

pages = "5704--5713",

journal = "The FEBS Journal",

issn = "1742-464X",

publisher = "Wiley-Blackwell",

number = "21",

}

TY - JOUR

T1 - Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.

AU - van Bloois, D.W.

AU - Koningstein, G.M.

AU - Bauerschmitt, H.

AU - Herrmann, J.M.

AU - Luirink, S.

PY - 2007

Y1 - 2007

N2 - Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. © 2007 The Authors.

AB - Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. © 2007 The Authors.

U2 - 10.1111/j.1742-4658.2007.06094.x

DO - 10.1111/j.1742-4658.2007.06094.x

M3 - Article

VL - 274

SP - 5704

EP - 5713

JO - The FEBS Journal

JF - The FEBS Journal

SN - 1742-464X

IS - 21

ER -