Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.

D.W. van Bloois, G.M. Koningstein, H. Bauerschmitt, J.M. Herrmann, S. Luirink

Research output: Contribution to JournalArticleAcademic

Abstract

Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. © 2007 The Authors.
Original languageEnglish
Pages (from-to)5704-5713
JournalThe FEBS Journal
Volume274
Issue number21
DOIs
Publication statusPublished - 2007

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Yeast
Escherichia coli
Saccharomyces cerevisiae
Membrane Proteins
Mitochondria
Mitochondrial Membranes
Electron Transport Complex IV
Chloroplasts
Eukaryota
Bacteria
Yeasts
Membranes
Proteins

Cite this

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title = "Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.",
abstract = "Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. {\circledC} 2007 The Authors.",
author = "{van Bloois}, D.W. and G.M. Koningstein and H. Bauerschmitt and J.M. Herrmann and S. Luirink",
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language = "English",
volume = "274",
pages = "5704--5713",
journal = "The FEBS Journal",
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}

Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC. / van Bloois, D.W.; Koningstein, G.M.; Bauerschmitt, H.; Herrmann, J.M.; Luirink, S.

In: The FEBS Journal, Vol. 274, No. 21, 2007, p. 5704-5713.

Research output: Contribution to JournalArticleAcademic

TY - JOUR

T1 - Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.

AU - van Bloois, D.W.

AU - Koningstein, G.M.

AU - Bauerschmitt, H.

AU - Herrmann, J.M.

AU - Luirink, S.

PY - 2007

Y1 - 2007

N2 - Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. © 2007 The Authors.

AB - Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. © 2007 The Authors.

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