Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 5704-5713 |
| Journal | The FEBS Journal |
| Volume | 274 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - 2007 |
Fingerprint
Cite this
}
Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC. / van Bloois, D.W.; Koningstein, G.M.; Bauerschmitt, H.; Herrmann, J.M.; Luirink, S.
In: The FEBS Journal, Vol. 274, No. 21, 2007, p. 5704-5713.Research output: Contribution to Journal › Article › Academic
TY - JOUR
T1 - Saccharomyces cerevisiae Cox 18 complements the essential Sec-independent function of Escherichia coli YidC.
AU - van Bloois, D.W.
AU - Koningstein, G.M.
AU - Bauerschmitt, H.
AU - Herrmann, J.M.
AU - Luirink, S.
PY - 2007
Y1 - 2007
N2 - Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. © 2007 The Authors.
AB - Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family. © 2007 The Authors.
U2 - 10.1111/j.1742-4658.2007.06094.x
DO - 10.1111/j.1742-4658.2007.06094.x
M3 - Article
VL - 274
SP - 5704
EP - 5713
JO - The FEBS Journal
JF - The FEBS Journal
SN - 1742-464X
IS - 21
ER -