Sample preparation for proteomics analysis: Filter-aided sample preparation (FASP) and single-pot solid-phase sample preparation (SP3)

Ka Wan Li*

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

The success of a proteomics experiment critically depends on the correct execution of the sample preparation. Gel-based sample preparation is a well-proven method widely used in qualitative and quantitative proteomics. Recently, sample preparation in a single vessel has gained popularity because of the ease to perform, the possibility for large-scale analysis, and the potential to improve sensitivity. In this chapter I describe two sample preparation protocols, filter-aided sample preparation (FASP) and single-pot solid-phase sample preparation (SP3).

Original languageEnglish
Title of host publicationNeuroproteomics
EditorsKa Wan Li
PublisherHumana Press Inc.
Pages67-73
Number of pages7
Edition2nd
ISBN (Electronic)9781493996629
ISBN (Print)9781493996612
DOIs
Publication statusPublished - 2019

Publication series

NameNeuromethods
Volume146
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • FASP
  • Proteomics
  • Sample preparation
  • SP3

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