Sampling and analytical strategies in on-line bioproces monitoring and control.

N.C. van de Merbel, H. Lingeman, U.A.T. Brinkman

Research output: Contribution to JournalArticleAcademicpeer-review


The use of on-line systems for the determination of small organic molecules during biotechnological processes is reviewed. Since sampling of part of the culture medium is an inherent and crucial part of the use of on-line systems, several sampling systems are described and compared and a categorization of sampling techniques - non-membrane, dialysis and ultrafiltration - is provided. Further, several analytical approaches - based on flow-injection or chromatographic analysis - and their relative merits for on-line measurements are discussed. Selected examples from the recent scientific literature serve to illustrate the applicability for on-line monitoring and control of bioprocesses.
Original languageEnglish
Pages (from-to)13-27
JournalJournal of Chromatography A
Publication statusPublished - 1996


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