TY - JOUR
T1 - Screening of dioxin-like toxicity equivalents for various matrices with wildtype and recombinant rat hepatoma H4IIE cells
AU - Behnisch, P.A.
AU - Hosoe, K.
AU - Brouwer, A.
AU - Sakai, S.
PY - 2002
Y1 - 2002
N2 - Determination of dioxin-like compounds utilizing in vitro bioassays such as ethoxyresorufin-O-deethylase (EROD) or chemical-activated luciferase expression (DR-CALUX®) is an important tool to evaluate their Ah receptor-mediated toxic effects. The aim of this study is to describe advantages and limitations of these bioassays using rat hepatoma (H4IIE) wildtype and recombinant H4IIE cells in a 96 well microtiter plate format. We are using these bioassays for the evaluation of relative responses (REP) from several congeners of dioxins (e.g., 2,3,4,7,8-PCDF) or dioxin-like compounds (PCB-126, 2,3,4,7,8-PBDF) to 2,3,7,8-TCDD. In addition, total toxic equivalency factors (TEFs) of mixtures of these dioxin-like compounds from several kinds of matrices such as feed, sediment, or thermal waste residues are measured by both bioassays and additional chemical analysis. These samples were measured in a cross-validation study between two laboratories using the DR-CALUX technology in comparison to the H4IIE-EROD assay and chemical analysis. Improvement of the quality criteria of the newly developed DR-CALUX bioassays in comparison to the EROD bioassay was demonstrated (higher coefficient of determination r
AB - Determination of dioxin-like compounds utilizing in vitro bioassays such as ethoxyresorufin-O-deethylase (EROD) or chemical-activated luciferase expression (DR-CALUX®) is an important tool to evaluate their Ah receptor-mediated toxic effects. The aim of this study is to describe advantages and limitations of these bioassays using rat hepatoma (H4IIE) wildtype and recombinant H4IIE cells in a 96 well microtiter plate format. We are using these bioassays for the evaluation of relative responses (REP) from several congeners of dioxins (e.g., 2,3,4,7,8-PCDF) or dioxin-like compounds (PCB-126, 2,3,4,7,8-PBDF) to 2,3,7,8-TCDD. In addition, total toxic equivalency factors (TEFs) of mixtures of these dioxin-like compounds from several kinds of matrices such as feed, sediment, or thermal waste residues are measured by both bioassays and additional chemical analysis. These samples were measured in a cross-validation study between two laboratories using the DR-CALUX technology in comparison to the H4IIE-EROD assay and chemical analysis. Improvement of the quality criteria of the newly developed DR-CALUX bioassays in comparison to the EROD bioassay was demonstrated (higher coefficient of determination r
U2 - 10.1093/toxsci/69.1.125
DO - 10.1093/toxsci/69.1.125
M3 - Article
SN - 1096-6080
VL - 69
SP - 125
EP - 130
JO - Toxicological Sciences
JF - Toxicological Sciences
IS - 1
ER -
