Screening white-rot fungi for bioremediation potential of 2,3,7,8-tetrachlorodibenzo-p-dioxin

Anh T.N. Dao, Jet Vonck, Thierry K.S. Janssens, Ha T.C. Dang, Abraham Brouwer, Tjalf E. de Boer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Ligninolytic fungi contain a number of representative strains consisting of mainly white-rot fungi (WRF) that produce lignin-modifying enzymes (LME) such as laccases and manganese peroxidases. Lignin-modifying enzymes are multipurpose enzymes which have potential for application in various fields such as, for example, bioremediation and biomass conversion. Because of the non-specific nature of these enzymes, they are also capable of biodegradation and removal of xenobiotic pollutants. In this study we used a tiered screening process where we screened over 70 Vietnamese WRF fungal isolates for LME activity and subsequently for the ability to breakdown the dioxin TCDD. After the initial screening we selected four fungal strains, which belong to the order of Polyporales, which excreted high laccase enzyme levels. The most active fungus being isolate FMD21, a species of Rigidoporus, which was isolated from a forest in the South of Vietnam and which produced both laccase and manganese peroxidase. In the optimized PDSRb medium, FMD21 laccase levels reaced activities of 238800 U/L after 10 days while MnP activity showed the highest activity at day 4 of aproximately 40 U/L. 2,3,7,8-TCDD, which is the most toxic dioxin congener, is a persistent organic pollutant of which few organisms are known that break it down. After the final screening, FMD21 was the only fungus capable of degrading TCDD and was able to reach a breakdown percentage of 73% after 28 days culture with a start concentration of 0.5 pg TEQ/μL TCDD. Co-cultivation experiments of up to three fungi were performed to test for a synergistic breakdown effect of TCDD but such an effect was not observed. FMD21 is a fungus that shows a potential to be used as a bioremediation agent to clean up dioxin contamination in the environment.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalIndustrial Crops and Products
Volume128
Early online date13 Nov 2018
DOIs
Publication statusPublished - Feb 2019

Fingerprint

tetrachlorodibenzo-p-dioxin
white-rot fungi
bioremediation
laccase
dioxins
screening
fungi
manganese peroxidase
enzymes
lignin
Rigidoporus
Polyporales
persistent organic pollutants
xenobiotics
biodegradation
Vietnam
pollution
pollutants
enzyme activity
biomass

Keywords

  • Dioxin breakdown
  • Ligninolytic enzymes
  • Mycoremediation
  • TCDD
  • White-rot fungus

Cite this

Dao, Anh T.N. ; Vonck, Jet ; Janssens, Thierry K.S. ; Dang, Ha T.C. ; Brouwer, Abraham ; de Boer, Tjalf E. / Screening white-rot fungi for bioremediation potential of 2,3,7,8-tetrachlorodibenzo-p-dioxin. In: Industrial Crops and Products. 2019 ; Vol. 128. pp. 153-161.
@article{e68da5c428c64c2887a021efd9af5aa7,
title = "Screening white-rot fungi for bioremediation potential of 2,3,7,8-tetrachlorodibenzo-p-dioxin",
abstract = "Ligninolytic fungi contain a number of representative strains consisting of mainly white-rot fungi (WRF) that produce lignin-modifying enzymes (LME) such as laccases and manganese peroxidases. Lignin-modifying enzymes are multipurpose enzymes which have potential for application in various fields such as, for example, bioremediation and biomass conversion. Because of the non-specific nature of these enzymes, they are also capable of biodegradation and removal of xenobiotic pollutants. In this study we used a tiered screening process where we screened over 70 Vietnamese WRF fungal isolates for LME activity and subsequently for the ability to breakdown the dioxin TCDD. After the initial screening we selected four fungal strains, which belong to the order of Polyporales, which excreted high laccase enzyme levels. The most active fungus being isolate FMD21, a species of Rigidoporus, which was isolated from a forest in the South of Vietnam and which produced both laccase and manganese peroxidase. In the optimized PDSRb medium, FMD21 laccase levels reaced activities of 238800 U/L after 10 days while MnP activity showed the highest activity at day 4 of aproximately 40 U/L. 2,3,7,8-TCDD, which is the most toxic dioxin congener, is a persistent organic pollutant of which few organisms are known that break it down. After the final screening, FMD21 was the only fungus capable of degrading TCDD and was able to reach a breakdown percentage of 73{\%} after 28 days culture with a start concentration of 0.5 pg TEQ/μL TCDD. Co-cultivation experiments of up to three fungi were performed to test for a synergistic breakdown effect of TCDD but such an effect was not observed. FMD21 is a fungus that shows a potential to be used as a bioremediation agent to clean up dioxin contamination in the environment.",
keywords = "Dioxin breakdown, Ligninolytic enzymes, Mycoremediation, TCDD, White-rot fungus",
author = "Dao, {Anh T.N.} and Jet Vonck and Janssens, {Thierry K.S.} and Dang, {Ha T.C.} and Abraham Brouwer and {de Boer}, {Tjalf E.}",
year = "2019",
month = "2",
doi = "10.1016/j.indcrop.2018.10.059",
language = "English",
volume = "128",
pages = "153--161",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier",

}

Screening white-rot fungi for bioremediation potential of 2,3,7,8-tetrachlorodibenzo-p-dioxin. / Dao, Anh T.N.; Vonck, Jet; Janssens, Thierry K.S.; Dang, Ha T.C.; Brouwer, Abraham; de Boer, Tjalf E.

In: Industrial Crops and Products, Vol. 128, 02.2019, p. 153-161.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Screening white-rot fungi for bioremediation potential of 2,3,7,8-tetrachlorodibenzo-p-dioxin

AU - Dao, Anh T.N.

AU - Vonck, Jet

AU - Janssens, Thierry K.S.

AU - Dang, Ha T.C.

AU - Brouwer, Abraham

AU - de Boer, Tjalf E.

PY - 2019/2

Y1 - 2019/2

N2 - Ligninolytic fungi contain a number of representative strains consisting of mainly white-rot fungi (WRF) that produce lignin-modifying enzymes (LME) such as laccases and manganese peroxidases. Lignin-modifying enzymes are multipurpose enzymes which have potential for application in various fields such as, for example, bioremediation and biomass conversion. Because of the non-specific nature of these enzymes, they are also capable of biodegradation and removal of xenobiotic pollutants. In this study we used a tiered screening process where we screened over 70 Vietnamese WRF fungal isolates for LME activity and subsequently for the ability to breakdown the dioxin TCDD. After the initial screening we selected four fungal strains, which belong to the order of Polyporales, which excreted high laccase enzyme levels. The most active fungus being isolate FMD21, a species of Rigidoporus, which was isolated from a forest in the South of Vietnam and which produced both laccase and manganese peroxidase. In the optimized PDSRb medium, FMD21 laccase levels reaced activities of 238800 U/L after 10 days while MnP activity showed the highest activity at day 4 of aproximately 40 U/L. 2,3,7,8-TCDD, which is the most toxic dioxin congener, is a persistent organic pollutant of which few organisms are known that break it down. After the final screening, FMD21 was the only fungus capable of degrading TCDD and was able to reach a breakdown percentage of 73% after 28 days culture with a start concentration of 0.5 pg TEQ/μL TCDD. Co-cultivation experiments of up to three fungi were performed to test for a synergistic breakdown effect of TCDD but such an effect was not observed. FMD21 is a fungus that shows a potential to be used as a bioremediation agent to clean up dioxin contamination in the environment.

AB - Ligninolytic fungi contain a number of representative strains consisting of mainly white-rot fungi (WRF) that produce lignin-modifying enzymes (LME) such as laccases and manganese peroxidases. Lignin-modifying enzymes are multipurpose enzymes which have potential for application in various fields such as, for example, bioremediation and biomass conversion. Because of the non-specific nature of these enzymes, they are also capable of biodegradation and removal of xenobiotic pollutants. In this study we used a tiered screening process where we screened over 70 Vietnamese WRF fungal isolates for LME activity and subsequently for the ability to breakdown the dioxin TCDD. After the initial screening we selected four fungal strains, which belong to the order of Polyporales, which excreted high laccase enzyme levels. The most active fungus being isolate FMD21, a species of Rigidoporus, which was isolated from a forest in the South of Vietnam and which produced both laccase and manganese peroxidase. In the optimized PDSRb medium, FMD21 laccase levels reaced activities of 238800 U/L after 10 days while MnP activity showed the highest activity at day 4 of aproximately 40 U/L. 2,3,7,8-TCDD, which is the most toxic dioxin congener, is a persistent organic pollutant of which few organisms are known that break it down. After the final screening, FMD21 was the only fungus capable of degrading TCDD and was able to reach a breakdown percentage of 73% after 28 days culture with a start concentration of 0.5 pg TEQ/μL TCDD. Co-cultivation experiments of up to three fungi were performed to test for a synergistic breakdown effect of TCDD but such an effect was not observed. FMD21 is a fungus that shows a potential to be used as a bioremediation agent to clean up dioxin contamination in the environment.

KW - Dioxin breakdown

KW - Ligninolytic enzymes

KW - Mycoremediation

KW - TCDD

KW - White-rot fungus

UR - http://www.scopus.com/inward/record.url?scp=85056473520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056473520&partnerID=8YFLogxK

U2 - 10.1016/j.indcrop.2018.10.059

DO - 10.1016/j.indcrop.2018.10.059

M3 - Article

VL - 128

SP - 153

EP - 161

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -